RESUMEN
The development of carbapenem-resistant Acinetobacter species is of serious concern in the hospital settings and naturally occurring oxacillinase genes (blaOXA) have been identified in several Acinetobacter species. In this study, we report the genome sequence of A. pittii TCM178 belongs to ST950, a multidrug-resistant isolate that harbored the blaOXA-72 and blaOXA-533 genes simultaneous. The genome size was estimated to be 3,789,564 bp with 3,501 predicted coding regions, and G+C content is 37.60%. Our findings have raised awareness of the possible constitution of a reservoir for peculiar carbapenemase genes in A. pittii that may spread among other Acinetobacter species in China.
Asunto(s)
Humanos , Proteínas Bacterianas/genética , Acinetobacter/efectos de los fármacos , Acinetobacter/enzimología , beta-Lactamasas/genética , Genoma Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple/genética , Acinetobacter/clasificación , Secuencia de Bases , China , Análisis de Secuencia de ADNRESUMEN
Abstract Worldwide increasing emergence of carbapenem-resistant Acinetobacter spp. has rendered the limited availability of effective antimicrobial agents and has become a major public health concern. In this study, we report the draft genome sequence of A. pittii TCM156, a multidrug-resistant isolate that harbored the blaOXA-357 gene. The genome sequence was further analyzed by various bioinformatics methods. The genome size was estimated to be 3,807,313 bp with 3508 predicted coding regions and G + C content is 38.7%. These findings have raised awareness of the possible emergence of OXA-type enzyme-producing A. pittii isolate in China.
Asunto(s)
Acinetobacter/genética , beta-Lactamasas/metabolismo , Infecciones por Acinetobacter/microbiología , ADN Bacteriano/química , Genoma Bacteriano , Análisis de Secuencia de ADN , Farmacorresistencia Bacteriana Múltiple , Composición de Base , Acinetobacter/aislamiento & purificación , Acinetobacter/efectos de los fármacos , Acinetobacter/enzimología , ADN Bacteriano/genética , ChinaRESUMEN
This study examined the association of pregnancy with urolithiasis and provided new insights into urolithiasis in pregnancy.A total of 462 subjects were studied from January 2004 to December 2009 in Foshan Maternal and Child Health Hospital,China.Among the 462 subjects,162 cases of urolithiasis during pregnancy (UPG) were selected as the observation group,150 cases of no urolithiasis during pregnancy (NUPG) served as pregnancy control group,and 150 cases of no pregnancy (NPG) at reproductive age who took part in physical examination were randomly assigned into non-pregnant control group.At the same time,the patients in observation group were divided into the following sub-groups:no symptomatic urinary calculus (NSUC) and symptomatic urinary calculus (SUC) groups;SUC group was further divided into surgical intervention (SI) and conservative management (CM) groups.The general information and the data of blood and urine were collected and compared among the groups.The results showed that the incidence of urinary calculi in pregnant women was lower than that in non-pregnant women,the formation of urinary stone was associated with the change of metabolism of protein and sugar in pregnant women,and the surgical intervention was a practicable alternative to treat the clinical intractable symptomatic urinary calculi in pregnancy.