Luteolin ameliorates loperamide-induced functional constipation in mice

Functional constipation (FC) is one of the most common gastrointestinal disorders characterized by hard stools and infrequent bowel movements, which is associated with dysfunction of the enteric nervous system and intestinal motility. Luteolin, a naturally occurring flavone, was reported to possess potential pharmacological activities on intestinal inflammation and nerve injury. This study aimed to explore the role of luteolin and its functional mechanism in loperamide-induced FC mice. Our results showed that luteolin treatment reversed the reduction in defecation frequency, fecal water content, and intestinal transit ratio, and the elevation in transit time of FC models. Consistently, luteolin increased the thickness of the muscular layer and lessened colonic histopathological injury induced by loperamide. Furthermore, we revealed that luteolin treatment increased the expression of neuronal protein HuC/D and the levels of intestinal motility-related biomarkers, including substance P (SP), vasoactive intestinal polypeptide (VIP), and acetylcholine (ACh), as well as interstitial cells of Cajal (ICC) biomarker KIT proto-oncogene, receptor tyrosine kinase (C-Kit), and anoctamin-1 (ANO1), implying that luteolin mediated enhancement of colonic function and contributed to the anti-intestinal dysmotility against loperamide-induced FC. Additionally, luteolin decreased the upregulation of aquaporin (AQP)-3, AQP-4, and AQP-8 in the colon of FC mice. In summary, our data showed that luteolin might be an attractive option for developing FC-relieving medications.

An experimental study of physical intelligence teaching on sensory integration function of 4-5-year-old children / 中国学校卫生

Objective@#To examine the impact of physical intelligence teaching on the function of children s sensory integration, so as to provide reference for promoting the development of sensory integration system.@*Methods@#From February to May 2023, the intervention was implemented for 12 weeks among 136 children aged 4-5 (68 in the intervention group and 68 in the control group). The intervention group received situational and game based physical intelligence teaching, the control group received sports game teaching according to the original curriculum objectives of the kindergarten. Intervention was administered 3 times a week for 40 minutes each time. The sensory integration ability of the intervention group and the control group were evaluated before and after the intervention with Chi square test and t test.@*Results@#The vestibular sensation, proprioception and tactile sensation of between boys and girls in the intervention group were significantly improved compared with before intervention (boys：44.14±11.52 vs. 53.34± 9.49 ，44.57±12.76 vs. 50.54±11.86，49.31±12.18 vs. 55.00±10.24,girls：46.00±11.01 vs. 54.58±10.06，48.79±13.17 vs. 53.64±11.97，52.67±11.67 vs. 56.91±10.42, t =-3.24，-2.49，-2.09，-5.24，-12.94，-2.56， P <0.05). There was no significant difference in vestibular sensation between boys and girls in the control group (boys：45.91±11.66 vs. 46.31± 11.20，girls：48.27±13.56 vs. 48.45 ±13.54, t =-0.87，-0.07， P >0.05), but there was a significant improvement in proprioception and tactile sensation in both boys and girls (boys：46.63±11.76 vs. 48.06±11.69，51.63±11.98 vs. 52.40±12.18，girls：50.45±12.16 vs. 51.67± 12.03 ，53.36±12.48 vs. 54.39±12.57， t =-3.36，-2.08，-4.66，-2.86， P <0.05). After the intervention, compared with the control group, the vestibular sensation of both boys and girls significantly improved ( t=2.83, 2.08, P <0.05), with exception of proprioception and tactile sensation ( t =0.88,0.67,0.97,0.88, P >0.05). In the experimental group, the number of normal boys increased from 12 to 24, while the number of dysfunctional boys decreased from 23 to 11, with a statistically significant difference ( χ 2=11.53, P <0.01). There was no statistically significant difference in sensory integration in boys of the control group before and after the experiment ( χ 2= 1.10 , P >0.05). After intervention,the number of normal girls in the experimental group increased from 15 to 27, while the number of dysfunctional girls decreased from 18 to 6, with a statistically significant difference ( χ 2=10.39, P < 0.05 ). There was no statistically significant difference in sensory integration in girls from the control group before and after the experiment ( χ 2=2.08, P > 0.05 ).@*Conclusion@#Physical intelligence teaching can effectively improve children s sensory integration ability, especially for vestibular function.

The pattern of alternative splicing and DNA methylation alteration and their interaction in linseed (Linum usitatissimum L) response to repeated drought stresses

BACKGROUND: Drought stress has significantly hampered agricultural productivity worldwide and can also result in modifications to DNA methylation levels. However, the dynamics of DNA methylation and its association with the changes in gene transcription and alternative splicing (AS) under drought stress are unknown in linseed, which is frequently cultivated in arid and semiarid regions. RESULTS: We analysed AS events and DNA methylation patterns in drought-tolerant (Z141) and drought-sensitive (NY-17) linseed under drought stress (DS) and repeated drought stress (RD) treatments. We found that the number of intron-retention (IR) and alternative 3' splice site (Alt3'SS) events were significantly higher in Z141 and NY-17 under drought stress. We found that the linseed response to the DS treatment was mainly regulated by transcription, while the response to the RD treatment was coregulated by transcription and AS. Whole genome-wide DNA methylation analysis revealed that drought stress caused an increase in the overall methylation level of linseed. Although we did not observe any correlation between differentially methylated genes (DMGs) and differentially spliced genes (DSGs) in this study, we found that the DSGs whose gene body region was hypermethylated in Z141 and hypomethylated in NY-17 were enriched in abiotic stress response Gene Ontology (GO) terms. This finding implies that gene body methylation plays an important role in AS regulation in some specific genes. CONCLUSION: Our study is the first comprehensive genome-wide analysis of the relationship between linseed methylation changes and AS under drought and repeated drought stress. Our study revealed different interaction patterns between differentially expressed genes (DEGs) and DSGs under DS and RD treatments and differences between methylation and AS regulation in drought-tolerant and drought-sensitive linseed varieties. The findings will probably be of interest in the future. Our results provide interesting insights into the association between gene expression, AS, and DNA methylation in linseed under drought stress. Differences in these associations may account for the differences in linseed drought tolerance.

The influencing factors of opening of college sports resources in the background of national public fitness services / Os fatores que influenciam a abertura de recursos esportivos universitários no contexto do serviço público de educação física nacional / Factores que influyen en la apertura de los recursos deportivos en las universidades

ABSTRACT There are some problems such as few and old equipment in urban community sports fitness facilities, which makes it difficult for community fitness resources to continue to meet the fitness needs of community residents. The opening of university sports resources can alleviate the problem of residents' fitness difficulty to a certain extent. In order to explore the problems faced by the opening of university sports resources, this study established the analytic hierarchy process model based on the analytic hierarchy process (AHP) and Delphi law in the background of national public service of fitness, and combined with the questionnaire survey method to study the influencing factors of opening of college sports. The model modified by the Delphi method contains 6 first-level and 22 second-level influencing factors. It is found that the most important factor in the first level is the hardware factor of college sports resources, and the most important factor of the second level is the spatial layout of university sports resources. The results show that improving the hardware facilities of university sports resources, improving the management mode of sports resources, and increasing the targeted financial subsidies and tax reduction and exemption are helpful to enhance the willingness to open sports resources and improve the quality of opened sports resources. It is hoped that this study can promote the opening of college sports resources and promote the continuous improvement of the service level of sports resources.

RESUMO Há alguns problemas, tais como equipamentos antiquados e em menores quantidades em instalações esportivas na Comunidade urbana, o que dificulta que os recursos de exercícios físicos continuem atendendo as necessidades de atividades físicas dos moradores das comunidades. A abertura de recursos desportivos universitários pode atenuar, em certa medida, o problema da dificuldade dos moradores de praticar atividades físicas. A fim de explorar os problemas enfrentados pela abertura dos recursos desportivos universitários, este estudo estabeleceu o modelo de processo hierárquico analítico baseado no processo hierárquico analítico (Analytic Hierarchy Process — AHP) e na Lei Delphi no contexto do serviço público nacional de atividades físicas, combinado com o método de inquérito por questionário para estudar os fatores que influenciam a abertura dos recursos esportivos universitários. O modelo modificado pelo método Delphi contém 6 fatores influenciadores de primeiro nível e 22 fatores influenciadores de segundo nível. Constata-se que o fator de primeiro nível mais importante é o fator físico dos recursos esportivos universitários, e o fator de segundo nível mais importante é a disposição espacial dos recursos esportivos universitários. Os resultados mostram que a melhoria das instalações físicas dos recursos desportivos universitários, a melhoria do modo de gestão dos recursos desportivos e o aumento dos subsídios financeiros e a redução e isenção de impostos são úteis para aumentar a disponibilidade da abertura dos recursos desportivos e melhorar a qualidade dos recursos desportivos abertos. Espera-se que este estudo possa promover o processo de abertura dos recursos esportivos universitários e promover a melhoria contínua do nível de serviço de recursos esportivos.

RESUMEN Explorar los problemas que enfrenta la apertura de los recursos deportivos universitarios, este estudio estableció el proceso de jerarquía analítica (PJA) y la ley Delphi en el contexto del servicio público nacional de educación física, y los combinó con la encuesta por cuestionario para estudiar los factores que influyen en la apertura de los deportes universitarios. El modelo modificado por el método Delphi contiene 6 factores de influencia de primer nivel y 22 de segundo nivel. Encontramos que el factor más importante en el primer nivel es el factor hardware de los recursos deportivos universitarios, y el factor más importante del segundo nivel es la distribución espacial de los recursos deportivos universitarios. Los resultados muestran que mejorar las instalaciones de hardware de los recursos deportivos universitarios, mejorar el modo de gestión de los recursos deportivos y aumentar los subsidios financieros específicos y la reducción y exención de impuestos son útiles para mejorar la disposición a abrir recursos deportivos y mejorar la calidad de los recursos deportivos abiertos. Esperamos que este estudio pueda promover la apertura de recursos deportivos universitarios y promover la mejora continua del nivel de servicio de los recursos deportivos.

Effects of non-steroidal anti-inflammatory drugs administered at different time points on the incidence of cystoid macular edema following phacoemulsification: a Meta-analysis / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To systematically evaluate the effects of non-steroidal anti-inflammatory drugs (NSAIDs)administrated at different time points on the incidence of cystoid macular edema (CME) following phacoem ulsification.METHODS:The Cochrane Library,PubMed,BMC,National Knowledge Infrastructure (CNKI),and VIP databases were searched to identify the clinical randomized controlled trials of comparing effects of NSAIDs administered at different time points on the incidence of CME and the central foveal thickness following phacoemulsification.The experiment group received topical NSAIDs preoperatively and postoperatively,while the control group received topical NSAIDs postoperatively.The RevMan software 5.2 and Stata software 12.0 were used in the Meta-analysis.RESULTS:Six studies were included in this Meta-analysis.No statistically differences were observed in the incidence of CME after 1wk postoperatively (OR=1.58,95％CI:0.48-5.18,P＞0.05),in the incidence of CME after 1 mo postoperatively (OR=0.78,95％CI:0.30-2.00,P＞ 0.05),in the central foveal thickness after 1wk postoperatively (WMD=-7.20,95％ CI:-15.17 to 0.77,P＞0.05),and in the central foveal thickness after 1mo postoperatively (WMD=-3.98,95％ CI:-14.05 to 6.08,P＞0.05).However,statistically significant differences were found in the incidence of CME after 3mo postoperatively (OR=0.22,95％ CI:0.11-0.43,P＜0.01)and in the central foveal thickness after 3mo postoperatively (WMD=-18.25,95 ％ CI:-33.80 to-2.70,P＜0.05).CONCLUSION:A combination of NSAIDs administrated preoperatively and postoperatively can reduce the incidence of the CME and the thickness of the macular centrall.Thereby,the effects of administrating NSAIDs both preoperatively and postoperatively have more advantages than that of administrating NSAIDs postoperatively alone.

Reversal of Multi-Drug Resistance by Vector-Based-ShRNA-Mdr1 In Vitro and In Vivo / 华中科技大学学报（医学）（英德文版）

In order to investigate the effects of vector-based hairpin small interference RNA (shRNA) on the reversal of multi-drug resistance (mdr) of A2780/Taxol cells, a novel vector pEGFP-H1/mdr1 containing mdr1-shRNA targeting at position 2943-2963 of mdr1 was designed and synthesized.Subsequently, A2780/Taxol cells were transfected with pEGFP-H1/mdrl, and the expression of mdr1 mRNA and P-gp was detected by using RT-PCR and Western blot respectively. MTT was used to measure the 50% inhibition concentration (1C50) of Taxol to A2780/Taxol cells. The results showed that at the 24th and 48th h after transfection, the expression of mdr1 mRNA was decreased to (52.1±1.0)% and (0.01+1.7)%, and that of P-gp decreased to (88.3±2.1)% and 0%, respectively. At the 48th h after transfection, the relative reversal rate of A2780/Taxol cells to Taxol was 69.54%. In vivo, the nude mice xenografts were injected with pEGFP-H1/mdrl, and then administrated Taxol.The tumor volume in pEGFP-H1/mdr1-transfected group was significantly reduced as compared with that in blank control group or pEGFP-H1-transfected group (807.20±103.16 vs 1563.78±210.54 or 1480.78±241.24 mm3, both P<0.01). These results suggested that transfection of pEGFP-H1/mdr1 could efficiently down-regulate the expression of mdr1 mRNA and P-gp in A2780/Taxol cells, and effectively restore the sensitivity of A2780/Taxol cells to Taxol both in vitro and in vivo.