RESUMEN
@#Human-animal parasitic diseases caused by medical helminths are hazardous to human health. Genetic polymorphism studies on medical helminth populations can not only understand the biological characteristics and genetic structure of their populations, but also help reveal how they adapt to their parasitic environment, thus contributing to deepen our understanding of the epidemiological patterns of parasitic diseases and improve our understanding of accurate prevention and control of parasitic diseases. With the development of molecular biology, molecular markers such as DNA barcodes, simple sequence repeats, and single nucleotide polymorphism markers have been widely used to study the genetic relationships among parasite populations and individuals, and to reveal the genetic variation of parasite populations and the evolution of species origins. In this paper, we systematically review the application of three molecular markers commonly used in the study of genetic polymorphism in medical helminths, with a view to laying the foundation for related research.
RESUMEN
Seven outbreaks of trichinosis occurred in Zhengzhou [Central China]. From 1992 to 1996, 250 patients were diagnosed. Trichinella larvae were found in 3 of 4 portions of the pork examined and in 5 of 7 cases by muscle biopsy. There was a higher occurrence of outbreaks in winter. Young and middle-aged workers, cadres and merchants were the majority of patients [higher in males than in females]. The most important clinical manifestations were fever, eyelid edema, tiredness and eosinophilia without any gastrointestinal symptoms, rash and with less myalgia. Anti-trichinella antibodies were detected by IF in 62.6% of individuals with the history of infection. The specific antibodies were only observed in 78% of patients one week after onset of disease, while the antibody positive rate was increased to 92.5%, 97.6% and 100% at 2, 3, 4 and 5 weeks after onset and evidently increased from 89.6% before therapy to 100% at 1 week after therapy, then decreased to 74.6% and 23.7% at 1 and 4 months after therapy, respectively. This suggested that IF is a useful tool for immunodiagnosis and checking up the therapeutic effect of trichinosis