Advance in Mendelsohn Maneuver for Dysphagia (review) / 中国康复理论与实践

Dysphagia can lead to multiple complications, increasing mortality and recurrence, and affecting the quality of life. Mendelsohn maneuver is a kind of physical therapy to improve the safety and effectiveness of swallowing. This paper introduced the origin of Mendelsohn maneuver, its physiological mechanism and clinical application. The potential risks and shortcomings of Mendelsohn maneuver were discussed, and a new solution was put forward.

Application of Standardized Patients Simulation with Microteaching in Instruction of Clinical Practice Skill of Rehabilitation Therapy / 中国康复理论与实践

Objective:To explore the effect of standardized patients stimulation combined with microteaching method in clinical teaching practice of students majored in rehabilitation therapy. Methods:From March, 2017 to March, 2018, 47 students majored in rehabilitation therapy in our department were selected with convenience sampling. Then they were randomly divided into control group (n = 23) and experimental group (n = 24). The control group received routine teaching method, while the experimental group received standardized patients stimulation combined with microteaching method. Their theoretical achievement, practical skills achievement and feedback on teaching effect were compared after this course. Results:The theoretical scores and practical skills scores were higher in the experimental group than in the control group (t > 2.366, P < 0.05). Meanwhile, the scores were higher in the experimental group than in the control group in the following aspects: the design capability of rehabilitation treatment program, the capability of selection of rehabilitation assessment tools, the communication and expression ability, learning enthusiasm and initiative, and the ability to relate theory to practice (t > 2.082, P < 0.05). Conclusion:Standardized patients simulation combined with microteaching method could improve the clinical practice effect of students majored in rehabilitation therapy.

Effects of Working Memory Updating Training on Verbal Fluency for Patients with Motor Aphasia / 中国康复理论与实践

Objective:To explore the effect of working memory updating training on verbal fluency for patients with motor aphasia. Methods:From June, 2015 to June, 2018, 80 patients with motor aphasia were randomly divided into control group (n = 40) and study group (n = 40). The control group accepted routine speech training, while the study group accepted working memory updating training in addition, for eight weeks. They were assessed updating function and verbal fluency before and after training. Results:The updating function and verbal fluency improved in the study group after training (t > 2.418, P < 0.05), and were better than those in the control group (t > 2.407, P < 0.05). Conclusion:Working memory updating training can improve both speech and cognition for patients with motor aphasia.

Advance in Air-pulse Stimulation for Dysphagia(review) / 中国康复理论与实践

Supraglottic and pharyngeal sensory abnormalities contribute to dysphagia and aspiration.Air-pulse stimulation makes the air pulse as stimulator to improve swallowing function. This paper reviewed some sorts of air-pulse stimulation equipment,in term of structure and operation,and its application in evaluation and training of swallowing function.

Design of electrode fixation and positioning cap for transcranial electrical stimulation / 医疗卫生装备

Objective To design a new type of rehabilitation device to execute electrode fixation and precision positioning during transcranial electrical stimulation.Methods The device was composed of a positioning cap,a soft rubber keel scaffold, an inlaid nut,a unionbolt,an electrical stimulation shim and etc.The electrode was put into the shim at the target site,and fixation and positioning were realized by regulating the depth of the bolt into the slot to change the attachment between the electrode fixing cushion and cranial skin,and then electrical stimulation therapy could be carried out reliably and accurately. Results The device gained advantages over the traditional means in fixation time in one time, dropping-off rate, patient satisfaction and easy operation.Conclusion This device behaves well in simple structure,easy manufacture,low cost,fixation and large applicability to kinds of population and electrical stimulators,and enhances the reliability of transcranial electrical stimulation therapy to some extent.

Protective effects of exogenous HS to the recovery of hypoxia post-conditioning on aged H9C2 cells and the underling mechanisms / 中国应用生理学杂志

OBJECTIVE@#To investigate the recovery of protective effects of exogenous hydrogen sulfide (HS) on hypoxia post-conditioning in aged H9C2 cells and its mechanism.@*METHODS@#H9C2 cells (cardiomyocytes line) were treated with 30 μmol/L hydrogen peroxide (HO) for 2 hours, then cultured for 3 days in order to induce cellular aging. Aged H9C2 cells were randomly divided into 5 groups (=8):Control group (Control), hypoxia/reoxygenation group (H/R), H/R + NaHS group, hypoxia post-conditioning (PC) group, PC+NaHS group. H/R model:the cells were exposed to hypoxic culture medium (serum and sugar free medium, pH=6.8) for 3 hours and then cultured at normal condition for 6 hours. PC model:at the end of hypoxia for 3 hours, the cells were exposed to normoxic culture solution for 5 minutes, then the cells were placed in hypoxic solution for 5 minutes, the cycle above-mentioned was repeated 3 times and followed by reoxygenation for 6 hours. Advanced glycation end products (AGEs) content and caspase-3 activity were detected by ELISA. The cell viability was observed by cell counting kit-8 (CCK-8). The reactive oxygen species (ROS) levels were analyzed using 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. The apoptotic rate was determined through Hoechst 33342 staining. The mRNA levels of relative gene expression were detected by real-time PCR.@*RESULTS@#Thirty μmol/L HO induced H9C2 cell senescence while did not lead to apoptosis. Compared with control group, cell viability was decreased, the apoptotic rate、levels of ROS and the mRNA of caspase-3, caspase-9 and Bcl-2 were increased in H/R and PC groups (<0.01). There were no differences in the above indexes between PC group and H/R group. Supplementation of NaHS increased cell viability and decreased apoptotic rate and oxidative stress. The effects of PC + NaHS on the above indexes were better than those of H/R+NaHS group.@*CONCLUSIONS@#Exogenous HS can restore the protective effect of PC on the aged H9C2 cells, and its mechanism is related to the inhibition of oxidative stress and apoptosis.

Protection effect and mechanism of hemin against ischemia/reperfusion injury in rat hearts / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate whether the cardioprotective effect of hemin against ischemia/reperfusion (I/R) injury is through the inhibition of calpain activity, and to explore its underlying mechanism.</p><p><b>METHODS</b>Sixty-four SD rats were randomly divided into eight groups (n = 8): sham, I/R, MDL+ I/R, MDL, hemin + I/R, hemin, and ZnPP + hemin+ I/R, ZnPP. Iangendorff isolated rat heart perfusion model was used. The rat hearts were suffered from 40 min of ischemia followed by 30 min of reperfusion. After that, left ventricular developed pressure (LVDP) was recorded. Infarct size and release of lactate dehydrogenase (LDH) were measured. Calpain, heme oxygenase (HO), and caspase 3 activities were evaluated. Expression of calpastatin protein was detected by Western blot.</p><p><b>RESULTS</b>(1) After suffered from ischemia/reperfusion, the calpain activity and caspase 3 activity increased. MDL28170, an inhibitor of calpain, prevented ischemia/reperfusion induced increases in LDH and infarct size, improved the LVDP recovery. (2) Compared with ischema/reperfusion rat hearts, pretreatment of hemin enhanced the HO-1 activity, decreased the calpain and caspase 3 activities, declined LDH release and infarct size, and improved LVDP recovery. (3) Ischemia/reperfusion reduced the expression of calpastatin protein in rat hearts, which was inhibited by hemin pretreatment. And HO-1 inhibitor could abolish the cardioprotection of hemin.</p><p><b>CONCLUSION</b>Cardioprotective effect of hemin against ischemia/reperfusion injury is through the inhibition of calpain activity, the mechanism might be involved in the increase in calpastatin protein expression.</p>

Protection of hypothermic preserved isolated rat hearts by resveratrol and its underlying mechanism / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To investigate whether resveratrol (RES) plays a protective role in hypothermic preserved isolated rat hearts and whether it is mediated by regulation of silent information regulator protein-1 (Sirt-1) expression.</p><p><b>METHODS</b>The Langendorff model of isolated rat heart was used. After stored in different Celsior solution at 4 degrees C for 9 h, SD rat hearts were randomly divided into 7 groups: blank control group;9 h group (soley hypothermic preservation for 9 h); RES group (3, 10, 30 micromol/L RES treatment plus hypothermic preservation for 9 h ), niacinamide (NAM) group (40 micromol/L NAM added in Celsior solution plus hypothermic preservation for 9 h), RES + NAM group (30 micromol/L RES and 40 micromol/L NAM were added in Celsior solution plus hypothermic preservation for 9 h). The morphological changes of cardiomyocytes were detected by the HE staining with the light microscope. The mRNA and protein expression levels of Sirt-1 were detected by Real-Time PCR and Western blot respectively.</p><p><b>RESULTS</b>(1) Compared with the blank control group, myocardiocytes were injured remarkably in the 9 h group and the Sirt-1 mRNA and protein expression levels were decreased significantly (P < 0.01); (2) Compared with the 9 h group, rat myocardial injury was alleviated gradually in 3, 10, 30 micromol/L RES group and the Sirt-1 mRNA and protein expression levels were increased in a dose-dependent manner (P < 0.05); (3) The above protective effects of RES were attenuated by Sirt-1 inhibitor NAM.</p><p><b>CONCLUSION</b>RES can protect myocardiocytes from injury caused by long range hypothermic preservation and this protective effect maybe mediated by upregulation of Sirt-1 expression.</p>

Expression of steroidogenic enzymes in the rat model of polycystic ovary syndrome / 生理学报

The aim of the present study was to investigate the expression changes of three steroidogenic enzymes in the polycystic ovary syndrome (PCOS). Thirty Sprague-Dawley (SD) rats were randomly divided into normal control (NC) group and PCOS group. PCOS rat model was established by DHEA injection. The serum levels of progesterone, estrogen and testosterone were measured by immunoradioassay or enzyme immunoassay. The cellular distributions of 3β-hydroxy steroid dehydrogenase (3β-HSD), 17β-hydroxy steroid dehydrogenase (17β-HSD) and cytochrome P450 aromatase (P450arom) in ovaries were detected by immunohistochemistry. The expression levels of 3β-HSD, 17β-HSD and P450arom were detected by RT-PCR and Western blot. The results showed that the serum levels of estrogen and testosterone of PCOS group were significantly higher than those of the NC group. There was no significant difference of serum progesterone level between the PCOS and NC groups. Compared with the NC group, the PCOS group showed increased mRNA and protein expressions of both 3β-HSD and 17β-HSD, as well as reduced P450arom mRNA and protein expressions. These results suggest that 3β-HSD and 17β-HSD, but not P450arom, may participate in the ovarian hormonal regulation in the present rat model of PCOS.