The anti-proliferation effect of total flavonoids of litchi seed combined with paclitaxel on prostate cancer paclitaxel resistant cells / 中国药理学通报

Aim To investigate the sensitization effect of total flavonoids of litchi seed (TFLS) to paclitaxel (PTX) on prostate cancer paclitaxel resistance (PCa-Txr) cells and the synergistic inhibitory effect of TFLS combined with PTX. Methods CCK-8 method was carried out to detect the inhibitory effect of TFLS and PTX on PCa-Txr cells as well as corresponding parental cells. PCa-Txr cells were either pre-treated or simultaneously treated with low cytotoxic dose of TFLS to observe the sensitivity of PCa-Txr cells to PTX. The synergistic inhibitory effect of TFLS combined with PTX on the proliferation of PCa-Txr cells were observed according to the method recommended by Chou T C. Combination index (CI) was used to determine whether TFLS and PTX had synergistic inhibitory effect. Results TFLS significantly inhibited the proliferation of PCa-Txr cells and parental cells in a certain time- and dose-dependent manner. The low dose of TFLS pre-treated or treated with PTX simultaneously failed to increase the sensitivity of PTX on PCa-Txr cells. TFLS combined with PTX significantly inhibited the proliferation of PCa-Txr cells with CI value less than one. Conclusions TFLS inhibits the proliferation of PCa-Txr cells, while TFLS combined with PTX has synergistic inhibitory effect on PCa-Txr cells. However, TFLS fails to increase the sensitivity of PCa-Txr cells to PTX.

Dissolution determination of Shuanghuanglian capsules by HPLC analysis assisted with principal component analysis / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a quality analysis method based on self-reference principal for dissolution determination of Shuanghuanglian capsules.</p><p><b>METHOD</b>Dissolution of Shuanghuanglian capsules was determined by principal component analysis consociated HPLC method.</p><p><b>RESULT</b>The liner of regression equation was good. The average recovery rates of quality assurance samples (QA) and quality control samples (QC) were all no less than 96. 0%. Dissolution curves of Shuanghuanlian capsules of different manufacturers and different batches of the same manufacturer had obvious disparity.</p><p><b>CONCLUSION</b>The method can better evaluate the dissolution conditions of Shuanghuanglian capsules. The prospect of the method is expected for assessing the dissolution of other oral solid dosage of traditional Chinese medicines.</p>