The value of MCV,MCH in the screening of thalassemia / 国际检验医学杂志

Objective To evaluate the value of the tests of mean corpuscular volume (MCV ) ,mean corpuscular hemoglobin (MCH) in the screening of thalassemia .Methods 603 cases were performed MCV and MCH tests and gene detection of thalasse-mia .The results of gene detection were used as reference standard .The sensitivity ,specificity ,accuracy ,positive predictive value and negative predictive value of single or combined results of MCV ,MCH tests were calculated .Results The sensitivity and specificity of single MCV test were 91 .2% and 76 .1% ,single MCH test were 91 .2% and 70 .7% .The sensitivity and specificity of parallel combined tests of MCV and MCH were 94 .8% and 61 .4% ,serial combined tests of MCV and MCH were 88 .4% and 75 .6% .Con-clusion The sensitivity of single MCV or MCH test in the screening of thalassemia is high .Parallel combined tests of MCV and MCH can improve the sensitivity ,and will be widely used in the in the screening of thalassemia .

Diagnosis and treatment of posterior fossa solid hemangioblastomas / 中国医师进修杂志

Objective To investigate the diagnosis and treatment of the posterior fossa solid hemangioblastomas (PFSHs).Methods The data of 23 patients with PFSHs verified by surgery and pathology were analyzed retrospectively.Results Nineteen cases were diagnosed with PFSHs before surgery.Total tumor removal was achieved in 22 patients.No case died of operation.A follow-up time was 0.33 -9.00 (2.96 ±2.73) years,20 patients returned to work,1 patient had self-handling living,and 2 patients died.Conclusions MRI and digital subtraction angiography are major preoperatively diagnostic modalities for PFSHs.PFSHs is still a kind of challenging neoplasms.Applicating special microsurgical technique and improving the operative manipulation can improve the surgical efficacy.

Studies on the extraction of DNA using carboxyl-coated magnetic particles and quantification using fluorescent dye / 生物医学工程学杂志

Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I, the quantification of double-strand DNA exhibits the advantages of high sensitivity,good repeatability and convenience.

Electrocorticography monitoring in microsurgical treatment of intracranial cavernous hemangiomas / 中国医师进修杂志

Objective To investigate the effects of electrocorticography(ECoG) monitoring in microsurgical treatment of intracranial cavernous hemangiomas(ICH).Methods Fifteen cases of ICH with secondary epilepsy were investigated from 2000 to 2005.They all had been performed ECoG monitoring during operation for determining the precise position and scope of focal epileptogenicity.Results Follow-up visit found 10 cases cured without drugs,4 cases total controlled with 1/4~1/3 quantity of premedicant,1 case had epileptic seizure by chance with drugs.Conclusion ECoG monitoring can provide the direction during the surgical procedure,and controls the postoperative epileptic seizure.

Expression and Significance of Interleukin-13 Receptor ?2 mRNA in Gliomas / 医学研究杂志

Objective To discuss the relationship between IL-13R?2 and the tumorigenesis and progression of gliomas. Methods Expression level of IL-13R?2 mRNA was studied by Reverse Transcription-Polymerase Chain Reaction(RT-PCR) in 20 cases of glioma and 5 cases of normal brain tissue. Results There was no expression of IL-13R?2 mRNA in all cases of normal brain tissue, wherea 13 of 20 gliomas expressed IL-13R?2.There were specially significant difference between them(P0.05) between low grade gliomas and high grade gliomas, there was greatly significant difference(P

Location of epileptogenic focus and selection of appropriate surgical methods in treatment of post-traumatic epilepsy / 中国医师杂志

Objective To evaluate the efficacy of combined various surgical procedures for the treatment of post-traumatic epilepsy.Methods According to the preoperative primary location of epileptogenic focus and the monitoring results of intraoperative electrocorticography and depth electrodes,21 seizure patients were treated with various surgical procedures: 7 cases with epileptogenic focus resection,8 cases with epileptogenic focus resection combined with multiple subpial transection(MST),4 cases with anterior temporal lobectomy combined with amygdalo-hippocampotomy,1 case with selective amygdalo-hippocampotomy under neuronavigation,and 1 case with anterior callosotomy combined with MST and bipolar coagulation on funtional cortexes. Results All the patients were followed up from 3 months to 10 years postoperatively,and the surgical outcomes were such as the followings: satisfactory outcome in 9 cases(42.9%),marked improvement in 7 cases(33.3%),improvement in 3 cases(14.3%),slight improvement in 1 case(4.8%) and no improvement in 1case(4.8%).There was no died cases caused by the surgical procedures.Eight patients had temporary dysfunction after operation,but all recovered in four weeks after surgery.Conclusion The combined various surgical procedures are effective and safe for the treatment of post-traumatic epilepsy.