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How to improve the performance of circulating tumor DNA (ctDNA) signal acquisition and the accuracy to authenticate ultra low-frequency mutation are major challenges of minimal residual disease (MRD) detection in solid tumors. In this study, we developed a new MRD bioinformatics algorithm, namely multi-variant joint confidence analysis (MinerVa), and tested this algorithm both in contrived ctDNA standards and plasma DNA samples of patients with early non-small cell lung cancer (NSCLC). Our results showed that the specificity of multi-variant tracking of MinerVa algorithm ranged from 99.62% to 99.70%, and when tracking 30 variants, variant signals could be detected as low as 6.3 × 10 -5 variant abundance. Furthermore, in a cohort of 27 NSCLC patients, the specificity of ctDNA-MRD for recurrence monitoring was 100%, and the sensitivity was 78.6%. These findings indicate that the MinerVa algorithm can efficiently capture ctDNA signals in blood samples and exhibit high accuracy in MRD detection.
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Humanos , Carcinoma de Pulmón de Células no Pequeñas/genética , Neoplasias Pulmonares/genética , Neoplasia Residual/patología , Biomarcadores de Tumor/genética , Biología ComputacionalRESUMEN
To compare carbon nanoparticles with methylene blue for sentinel lymph nodes mapping in laparoscopic staging of early endometrial carcinoma.Forty five patients with stage I or stage Ⅱ endometrial carcinoma undergoing laparoscopic operation from January 2016 to January 2018 were randomly divided into two groups:methylene blue was applied in 20 cases and carbon nanoparticies were applied in 25 cases for sentinel lymph node (SLN) mapping in laparoscopic staging;and the images of two tracers were compared.The development time of nano-carbon group was shorter[(16.4±3.4) min vs.(21.0±5.9)min,t=3.26,P=0.02] and duration was longer [(73.1 ± 14.3) min vs.(54.6 ± 14.9)min,t=5.41,P=0.04] than those of methylene blue group.In lymphatic mapping,the detection rate of SLN in nano-carbon group was significantly higher than that of methylene blue group [95％ (21/22)vs.5/18].For detection of metastatic SLN,the sensitivity of methylene blue group was 1/2 and that of nano-carbon group was 4/4 (x2=66.70,P＜0.01).The results indicate that both methylene blue and Nano-carbon tracer can be used for SLN mapping in laparoscopic staging of early endometrial carcinoma,and nano-carbon tracer is recommended as the first choice to predict pelvic lymph node metastasis in endometrial carcinoma surgery.
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Objective To explore the role of MR stem cell markers in temozolomide intervention gliomas angiogenesis and therapy .Methods C6 glioma cells were intravenously injected in glioma model rat.MR stem cell markers and immunohistochemistry were used to observe the tumor growth and angiogenesis.Correlation of contrast imaging and immunohistochemistry results were studied.Results In this study, MR scanning was successfully used to trace the SPIO-PLL marked bone marrow stem cells, dynamic testing of angiogenesis in rat brain glioma tumor.Correlation analysis of imaging and immunohistochemical showed that they had a good negative correlation (P<0.05).Conclusion SPIO magnetic markers for bone marrow stem cells and MR tracing technique is an effective means of dynamic observation in vivo glioma angiogenesis.
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Objective To inuestigate the clinical efficacy of complete dentures combined with Naoluotong capsule on patients with edentulous . Methods Edentulous patients were given dentures and denture combined with Naoluotong capsule therapy for the treatment.After treatment, chewing efficiency, cerebral blood flow and radiographic were tested and evaluated.Results After 3 months of treatment, satisfaction of combined treatment group was 90.00% which were significantly higher than that of complete denture group(P<0.05); chewing efficiency and cerebral blood flow(Vs、Vm、Vd) results were significantly higher than the denture group (P<0.05);MR studies showed that combined treatment can make the level of activation in the brain regions of patients with missing teeth to the normal value .Conclusion Denture combined with Naoluotong capsule treatment for edentulous patients can get better clinical outcomes and improve brain function of the patients.
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Objective To observe the effects of compound lidocaine cream on patients with tracheotomy during recovery from general anesthesia.Methods Fifty-four patients scheduled for radical oral cancer resection and tracheotomy under general anesthesia were randomly allocated into two groups:control group (group C,27 patients) and compound lidocaine cream group (group L,27 patients).Compound lidocaine cream was uniformly smeared on the airway segment of tracheotomy tube in group L,and no drug was coated on tracheotomy tube in group C.Hemodynamic parameters before induction (To),at the end of surgery(T1),at the moment of awake(T2) and 1 min after awake(T3),respiration recovery time,awake time,time spent in the postanesthetic care unit (PACU),coughing,and agitation after surgery were monitored.Results There were no difference in the operation time,sevoflurane inhale time,respiration recovery time and awake time between two groups (P ＞ 0.05).The time spent in the PACU in group L was less than that in group C [(27.3 ± 6.9)min vs.(34.9 ± 6.6) min] (P ＜ 0.05).The HR and MAP of T3 in group C were significantly higher than those ofT0 [(113.5 ± 18.0) bpm vs.(82.2 ± 13.1) bpm,(112.7 ± 15.7) mmHg (1 mmHg =0.133 kPa) vs.(87.5 ± 12.7) mmHg] (P ＜ 0.01).The HR and MAP of T3 in group L were lower than those in group C [(92.6 ± 12.8) bpm vs.(113.5 ± 18.0) bpm,(98.3 ± 13.5) mmHg vs.(112.7 ± 15.7) mmHg] (P ＜ 0.01 or P ＜ 0.05).Degree of coughing in group L was lower than that in group C (Z =-2.409,P ＜ 0.05).Degree of agitation in group L was lower than that in group C (Z =-2.013,P ＜ 0.05).Conclusion Topical anesthesia of tracheal mucosa using compound lidocaine cream can effectively alleviate hemodynamics fluctuations,coughing and agitation after tracheotomy during recovery for general anesthesia.
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Optical coherence tomography (OCT) has developed from time-domain into Fourier-domain OCT (FD-OCT) which indicates clearer details and higher resolution of images. FD-OCT can indicate the structure and pathological changes of each retinal layer, and reveal the retinal external limiting membranes and changes of inner-and outer-segment of visual cells by 3D solid reconstruction. FD-OCT not only provide detailed information of the images for the clinical diagnosis, but also help us investigting the characteristics and pthological mechanisms of ocular fundus diseases, which lead us to a new era of technology of observation on ocualr fundus diseases. In the application, we should pay attention to the significance of different colors of OCT images, and focus on the cohenrence of the position in the image acquistion during the follow-up period. Dynamic observation on the lesions by FD-OCT and aggregated anaylsis of resutls of several imageological examination would be the development direction of imageological examination of ocular fundus diseases.
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Objective To investigate the value of application of multi-slice helical CT(MSCT) indiagnosis of basicranial fracture. Methods Sixty-two patients with basicranial fracture were scanned withthin-section scanning mode after conventional CT scanning The images were transferred to the work-station for reconstruction including multiplanar reconstruction (MPR), volume rendering (VR). Comparison between thin-section scanning images and conventional CT scanning images were performed. Results Compared with the conventional CT scanning (67.9% and 45.0% respectively),the rates of fracture displayed of images acquired from thin-section scanning in diagnosis of fractures of anterior and middle cranial fossa (100.0% and 95.0% respectively) ,P＜0.01. The two scanning mode in diagnosis of fractures of posterior cranial fossa( 88.9% and 100.0% respectively )differed from each other unstatistically(P＞0.05 ). ConclusionThe thin-section scanning of MSCT can improve the diagnostic correctness of basicranial fracture.
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Objective To analyze the clinical applicative value of treating ectopic pregnancy with laparoscopy.Methods The clinical datas of 125 cases ectopic pregnancy were treated by operational laparoscopy.Extruding gestational sac(GS) in fimbria,taking embryo with opening fallopian tube,salpingectomy with using laparosope,and comparing with abdominal salpingectomy.Results The mean operating time was (93.3?10.6)min,the mean duration of hospitalization after operating was (4.3?1.0)d,laparosopic surgery had an obvious difference with abdominal salpingectomy(P