RESUMEN
OBJECTIVE: To prepare matrine solid lipoid nanoparticle,establish preparating method and determine the encapshlation efficiency. METHODS: Matrine solid lipoid nanoparticle was prepared by microemulsion-probe ultrasonic method and its quality was evaluated by particle size, Zeta potential, microscopic morphology and in vitro release. The encapsulation efficiency of the carrier was measured by different methods and their effect was compared. RESULTS: The diameter of matrine solid lipoid nanoparticle was (116.7±2.6) nm and its Zeta potential was (-45±1.7)mV. Transmission electron micrographs showed that the solid lipoid nanoparticle was uniform in size and spherical. The in vitro release result suggested the carrier exhibited control release character. Dextran gel microcolumn centrifugation can effectively separate free drugs and carriers, and the measured encapsulation efficiency data has little difference in stability. CONCLUSION: Matrine solid lipoid nanoparticle is successfully prepared and their particle size, Zeta potential and in vitro release quality are evaluated.Dextran gel microcolumn method is effective in the measurement of matrine solid lipoid nanoparticle, providing a reliable reference for the determination of water-soluble drug encapsulation efficiency.