Multi-indicators rapid determination in alcohol precipitation of Lonicerae Japonicae Flos and Artemisiae Annuae Herba in Reduning Injection by near-infrared spectroscopy / 中草药

Objective: Near-infrared (NIR) spectroscopy was used to rapidly determine the concentration of chlorogenic acid (CA) and soluble solid content in alcohol precipitation of Lonicerae Japonicae Flos and Artemisiae Annuae Herba (LA) in Reduning Injection, so as to increase the level of quality control of alcohol precipitation process. Methods: The LA samples of alcohol precipitation were collected. The PLS algorithm was used to establish NIR models between spectra and concentration of CA and total solid content, and the concentration of CA and soluble solid content in unknown batch were predicted. Results: The correlation coefficients (R) of NIR models were above 0.980 in calibration sets. The R of verification model was above 0.980, the root mean square error of prediction (RMSEP) for CA and soluble solid content were 0.541 and 0.218, respectively, and the relative standard errors (RSEP) for CA and soluble solid content were no more than 8%. Conclusion: The results demonstrate that NIR could determine the concentration of CA and soluble solid content in alcohol precipitation of Reduning Injection rapidly and accurately.

Gene chip analysis of mutation of HBV-DNA open reading frame / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To detect the mutation of HBV-DNA open reading frame (ORF), provide evidences for clinical diagnosis and treatment.</p><p><b>METHODS</b>By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF.</p><p><b>RESULTS</b>The mutations of HBV-DNA ORF existed widespread. The rates of mutation on Pre-C 1896, PreC 1814, BCP 1762, BCP 1764, P 528, P 552MI, P 552MV were 23.5%, 3.9%, 55.9%, 53.9%,39.2%, 38.2%, 10.8%, respectively.</p><p><b>CONCLUSION</b>The gene chip technique possesses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation. The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.</p>

Study on the expression of Toll-like receptor 4 in peripheral blood mononuclear cell of patients with chronic hepatitis B virus infection / 中华传染病杂志

Objective To examine the expression of Toll-like receptor(TLR)4 protein and the transcription of TLR4 mRNA in the peripheral blood mononuclear cells(PBMC)from patients with hepatitis B virus(HBV)infection and explore the relationship between TLR4 and chronic HBV infec- tion.Methods The expression level and transcription level of TLR4 were determined by flow cytometre and reverse transcriptase polymerase chain reaction respectively in PBMC from 37 chronic hepatitis patients,28 liver cirrhosis patients,31 severe hepatitis patients and 27 healthy controls. Meanwhile,liver function,as well as blood routine test,prothrombin test activity(PTA)and HBV DNA was measured.Results The expression level and transcription level of TLR4 in patients were higher than those in healthy controls(P