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Objective To explore the effect of gastrin_(17) on E cadherin/?-catenin complex,a down- stream effector of FAK pathway,in colonic carcinoma cell line CoLo320WT.Methods pCR3.1/GR plasmid expressing gastrin receptor CCK 2R was transfected into colonic carcinoma cell line CoLo320 by Lipofectamine~(TM) 2000 and expressing stably CCK 2R clones was screened by G418.The expression levels of gastrin receptor of Coi.o320 and the transfected cell line Colo320WT were assayed by RT PCR. CoLo320WT cells were treated by 10~(-8)mmol/L gastrin_(17) at distinct time points (0 hr,1 hr,6 hr,12 hr, 24 hr,48 hr),whilst treated by 10_(-6) mmol/L L365,260 (gastrin_(17) receptor blocker) simultaneously for 30 minutes and then treated by gastrin_(17) again for 12 hr.Expression levels of phosphorylated FAK Tyr397 and total FAK in CoLo320WT under gastrin_(17)intervention were detected hy Western blot.Ex- pression levels of E-cadherin and?-catenin complex in TX-100 solution fraction and TX-100 insolution fraction of CoLo320WT cells were detected by coimmunoprecipitation and Western blot.Distribution of E-cadherin and?-catenin in CoLoWT320 were observed by immunocytochemistry.Results Phosphoryla- ted FAK Tyr397 expression in CoLo320WT cells increased in time dependent fashion under gastrin_(17) intervention and peaked at 12 hour after intervention,while decreased by L365,260 inhibition.But gas trine_(17) had no effect on total FAK in CoLo320WT cells.Expresion levels of E-cadherin and?-catenin com- plex in TX-100 solution fraction were decreased apparently,but increased again after L365,260 block- ing.On the contrary,the expression levels of E-cadherin and?-eatenin complex in TX-100 solution frac tion differed from that in TX-100 solution.Cytoehemistry observation had revealed that E-cadherin and?-catenin transferred from cell membranes into cndochylemas,nuclei and cytoskeleton under gastrin_(17) in- tervention.Conclusions Gastrin_(17)affected significantly the distribution of E cadherin/?-catenin complex in CoLo320WT by phosphorating FAK Tyr397 and activating FAK pathway when it bound to its recep- tor CCK-2,therefore promoted invasion and metastasis of CoLo320WT.
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AIMTo evaluate the influence of serum UA level and ren al function of hyperuricemic rats administrated Hus. METHODSHype ruricemia and uric acid nephropathy in rats were induced by adenine ig feeding f eeds containing 10% yeast Drug therapy was given Simultaneously. After 18 d, blo od of rats was drawn from the hearts. Total protein, albumin(A), A/G ratio, uric acid(UA), creatinine(Cr), urea nitrogen(BUN), triglycerides (TG), high density lipoprotein (HDL), and Cholesterol of serum were detected by automatic biochemis try analyzer. Ultrastructural alteration and UA crystal were observed. R ESULTSSerum UA, Cr, BUN of Hus groups and All group were significantly r educed compared with model group(P
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Histamine H 1R antagonists are mainly administered to treat the diseases of hypersensitivity reaction. And histamine H 2R antagonists are mainly administered to treat gastroenteric diseases. But in recent years administered simultaneously H 1R and H 2R antagonists can enhance their effects. Combination of H 1R and H 2R antagonists has good therapeutic effect on hypersensitivity reaction, cancers, asthma, etc, and can eliminate side effects.