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Objective To explore the biological exposure limit of blood system damage caused by long-term exposure to polycyclic aromatic hydrocarbons (PAHs) in non-occupational population by using the benchmark dose method, and to provide relevant reference for further improving the assessment of PAHs-induced health damage effects. Methods Adult residents living in downwind direction of a coke-oven plant in Shanxi Province were selected as the research subjects, and the information collected from baseline was used as the control. The metabolites of PAHs in urine were used as exposure biomarker, and the abnormal rate of red blood cell index was used as response biomarker. The relationship between urinary OH-PAHs and the erythrocyte damage rate was analyzed, and the benchmark dose (BMD) and the lower confidence limitation for the benchmark dose (BMDL) were calculated using Bayesian dose-optimizing software. Results The urinary PAH metabolites were mainly naphthalene and fluorene. The detection concentrations of 2-OHFlu and 1-OHPhe in the final year were higher than those in the baseline (P<0.05). With the increase of exposure years, the abnormal rate of red blood cells in the final year was higher than that in the baseline (P<0.05). In addition, the abnormal rate of red blood cells increased with the increase of the concentrations of five metabolites of PAHs in urine, and the chi-square trend test was significant (P<0.05). The benchmark dose (BMD) of OH-PAHs was 0.67 μmol/mol Cr, 0.82 μmol/mol Cr, 1.40 μmol/mol Cr and 0.78 μmol/mol Cr, respectively. The BMD of 2-OHNap in people with barbecue diet habits was 0.23 μmol/mol Cr. The BMD of 2-OHNap in people without barbecue diet habits was 1.44 μmol/mol Cr. Conclusion There is a dose-response relationship between the concentration of PAHs metabolites in urine and the damage of red blood cells. Long-term exposure to PAHs can lead to hematological damage. It is suggested that targeted public health interventions should be formulated to reduce the exposure of the general population to PAHs.
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Objective To compare the clinical value of alpha-fetoprotein (AFP), chemokine (C-C motif) ligand 2 (CCL2) and haptoglobin (HP) in the diagnosis of liver metastasis of colorectal cancer. Methods 256 patients with colorectal cancer in the 983rd Hospital of the Joint Service Support Force of PLAl from Jan. 2016 to Dec. 2018 and 92 healthy subjects (control group) were enrolled. The patients were grouped according to liver metastasis, including liver metastasis group (72 cases) and non-liver metastasis group (184 cases). The serum levels of AFP, CCL2 and HP in different groups were compared. The diagnostic values of AFP, CCL2 and HP in liver metastasis of colorectal cancer were analyzed by ROC curve. Results The serum concentrations of AFP, CCL2 and HP were increased in the control group, non-liver metastasis group and liver metastasis group in order, and the diffences between any two groups were statistically significant (all P<0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The results of diagnostic efficacy for colorectal cancer liver metastases showed that AFP+CCL2+HP had the highest sensitivity (93.05％), HP had the highest specificity (92.75％), CCL2 +HP had the hightest Judden index (0.80), HP had the highest positive predictive rate (75.60％), and AFP + CCL2+HP had the hightest negative predictive rate (97.92％). Conclusions Early diagnosis and prevention of liver metastasis of colorectal cancer can improve the long-term survival rate of patients with colorectal cancer. HP, CCL2 and AFP have important clinical value in early diagnosis of liver metastasis of colorectal cancer.
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Objective@#To compare the clinical value of alpha-fetoprotein (AFP), chemokine (C-C motif) ligand 2 (CCL2) and haptoglobin (HP) in the diagnosis of liver metastasis of colorectal cancer.@*Methods@#256 patients with colorectal cancer in the 983rd Hospital of the Joint Service Support Force of PLAl from Jan. 2016 to Dec. 2018 and 92 healthy subjects (control group) were enrolled. The patients were grouped according to liver metastasis, including liver metastasis group (72 cases) and non-liver metastasis group (184 cases). The serum levels of AFP, CCL2 and HP in different groups were compared. The diagnostic values of AFP, CCL2 and HP in liver metastasis of colorectal cancer were analyzed by ROC curve.@*Results@#The serum concentrations of AFP, CCL2 and HP were increased in the control group, non-liver metastasis group and liver metastasis group in order, and the diffences between any two groups were statistically significant (all P<0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The difference in CEA between two experimental groups was not statistically significant (P>0.05). The results of diagnostic efficacy for colorectal cancer liver metastases showed that AFP+CCL2+HP had the highest sensitivity (93.05%), HP had the highest specificity (92.75%), CCL2+HP had the hightest Judden index (0.80), HP had the highest positive predictive rate (75.60%), and AFP+CCL2+HP had the hightest negative predictive rate (97.92%).@*Conclusions@#Early diagnosis and prevention of liver metastasis of colorectal cancer can improve the long-term survival rate of patients with colorectal cancer. HP, CCL2 and AFP have important clinical value in early diagnosis of liver metastasis of colorectal cancer.
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Objective To investigate stilbene glycosides(TSG) and PNS concomitantly on PC12 cell survival rate of Alzheimer's disease.Methods The nerve cells that were seeded on the two culture plates were cultured for 1 day after the removal of primary culture fluid.In addition to the blank group, the model group and drug compatibility group were added 5 μl Aβ25-35 perpore to induced PC12 cell damage.To established AD cell damage model after exposure to the circumstances for 24 hours.Uniform design and factorial design were used respectively.After 1 d, using MTT method in ELISA analyzer measured the OD value of each pore, and calculating the survival rate of cells.Results The uniform design results showed that the cell survival rate was significantly linear with TSG and PNS (P＜0.05).From the equation, The higher the dose, the higher the cell survival rate.In this experimental condition, TSG and PNS respectively 50 mg/L, 200 mmol/L achieved the highest cell survival rate.2×2 factorial design experiments showed that, compared with the model group, the cell survival rate of TSG-PNS group (74.46％ ± 2.06％ vs.65.42％ ± 1.42％) increased (P＜0.05), but there was no interaction between the two groups (P=0.053).This showed that the combination of the two drugs in this dose has a protective effect on AD damage.Conclusion The compatibility of total saponins of two stilbene glucoside and three seven combination has the synergistic effect of anti AD damage.