RESUMEN
Alzheimer's disease (AD), also called presenile dementia, is one of the most common neurodegenerative diseases in elderly people. The main pathological features of AD include senile plaques (SPs), neurofibrillary tangles (NFTs) and neuron loss. A biomarker is a characteristic that can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacological responses to a therapeutic intervention. Class biomarkers of AD such as Abeta and phosphorylated tau have been widely used in clinical diagnosis of AD patients. Recently, novel technologies like proteomics, genomics, and imaging techniques have expanded the role of a biomarker from early diagnosis to monitoring the progression of diseases and evaluating the response to various treatments. In this article, we will review the progress of various biomarkers of AD.
Asunto(s)
Humanos , Adipoquinas , Líquido Cefalorraquídeo , Enfermedad de Alzheimer , Líquido Cefalorraquídeo , Diagnóstico , Diagnóstico por Imagen , Metabolismo , Péptidos beta-Amiloides , Líquido Cefalorraquídeo , Biomarcadores , Proteína 1 Similar a Quitinasa-3 , Fluorodesoxiglucosa F18 , Lectinas , Líquido Cefalorraquídeo , Fragmentos de Péptidos , Líquido Cefalorraquídeo , Fosforilación , Tomografía de Emisión de Positrones , Presenilinas , alfa 1-Antitripsina , Sangre , Proteínas tau , Líquido CefalorraquídeoRESUMEN
One common feature of glaucoma, optic neuritis and some other optic nerve diseases is sustained and irreversible apoptosis of retinal ganglion cells (RGCs). Ginkgolide B is believed to protect neurons in brain and contribute to neurite outgrowth and synapse formation. The aim of the present study was to explore the effects of Ginkgo biloba extract (EGB761) and ginkgolide B on axonal growth of RCGs. Retina explants were cultured in three-dimensional tissue culture system, and the number and length of neurites were analyzed. Immunohistochemistry staining was performed to confirm that the neurite observed was axon of RGCs. TUNEL and activated caspase-3 staining were also applied to observe RGCs apoptosis. The result shows that neurites of RGCs treated with EGB761 or ginkgolide B were more and longer than those in control. The neurite is proved to be the axon of RGCs by immunostaining. Furthermore, compared with control group, RGCs treated with ginkgolide B showed decreased cellular apoptosis and inhibited caspase-3 activation. These results suggest ginkgolide B can promote RGCs axon growth by protecting RGCs against apoptosis.