RESUMEN
Objective To investigate the expression and significance of E-cadherin and β-catenin in type Ⅰ endometrial carcinoma.Methods The expression of E-cadherin and β-catenin were detected by immunohistochemistry in 60 patients with type Ⅰ endometrial carcinoma and 10 normal endometrial tissues.The patients were followed-up postoperatively for the survival rate.Results Expression of E-cadherin and β-catenin in 10 normal endometrial tissues was all high expression,while those of both abnormal expression in type Ⅰ endometrial carcinoma with expression rates with 60.0 ％ (36/60) and 55.0 ％ (33/60),respectively,which were difference compared with both in normal endometrial tissues (P ＜ 0.05).The expression rates were markedly decreased in carcinoma with lymph node metastasis than those without lymph node metastasis.The proportions of abnormal expressions of E-cadherin and β-catenin were positively correlated with tumor grade and myometrial invasion in type Ⅰ endometrial carcinoma (P ＜ 0.05).The postoperative two-year survival rate in the patients with abnormal expression of E-cadherin and β-catenin were much lower than those with normal expression (P ＜ 0.05).Conclusions Abnormal high expression of E-cadherin and β-catenin may play important roles on generation and metastasis in type Ⅰ endometrial carcinoma.Both of them may be promising method for predicting invasion and metastasis and can be prognostic predictors in type Ⅰ endometrial carcinoma.
RESUMEN
Objective To investigate the effect of Brassica rapa L-Polysaccharide on inflammatory response with asthmatic rats.Methods Forty Wistar rats were randomly divided into normal control group,model group,low-dose group and turnip large-dose group of Brassica rapa L.extract.The rats of model group,low-dose group and turnip large-dose group of Brassica rapa L-Polysaccharide were made into the model of asthma.The rats of low-dose group and turnip large-dose group of Brassica rapa L-Polysaccharide were given Brassica rapa L-Polysaccharide from gavage with 5 mg/kg and 2.5.mg/kg,and the rats of normal control group and model group were given distilled water,lasting two weeks.The serum inflammatory factor levels and bronchoalveolar lavage fluid (BALF) cell number of each group of asthmatic rats was detected.Results The serum levels of TNF-alpha,IL-6,IL-2 and CRP of low-dose and high-dose groups of asthmatic rats were significantly lower than the model control group[(90.33 ± 5.90)pg/ml,(92.01 ± 3.26)pg/ml vs (136.43 ± 7.81) pg/ml; (19.08 ± 1.27) pg/ml,(18.33 ± 2.09) pg/ml vs (25.14 ± 2.31) pg/ml;(115.36 ± 7.27) pg/ml,(110.72 ± 10.05) pg/ml vs (193.16 ± 12.33) pg/ml ; (70.58 ± 14.85) μg,/ml,(71.69 ± 12.11) μg/ml vs (93.21 ± 17.25) μg/ml],with a statistically significant difference (t =3.144～ 17.188,P ＜0.01) ; The total cells,eosinophils ratio and proportion of BALF lymphocytes of low-dose and high-dose groups of asthmatic rats were significantly lower than the control group[(1.58 ± 0.67) × 109/L,(1.55 ±0.89)×109/L vs (3.05 ±1.21) × 109/L;(20.31 ±4.27)％,(21.06±3.53)％ vs (26.35± 5.91) ％ ; (16.77 ± 5.47) ％,(15.89 ± 6.03) ％ vs (23.56 ± 4.81) ％],with a statistically significant difference(t =2.430 ～ 3.158,P ＜ 0.05,P ＜ 0.01).Conclusions The Brassica rapa L.Polysaccharide can effectively inhibit the secretion of various inflammatory factors in rats of asthma,and inflammatory response occurred.