RESUMEN
Objective To investigate the effects of arsenic trioxide(ATO) on anti-dsDNA antibody and lymphocyte subsets proliferation in splenic cells of MRL/lpr mice.Methods MRL/lpr mice were divided into three groups: ATO group(0.4 mg/kg?d,ip),sodium chloride(NS) group(0.25 mL,ip),and cyclophosphamide(CTX) group(50 mg/kg?qw,ip).The control group consisted of 12 syngeneic normal C57/BL mice,which were sub-divided into ATO group(0.4 mg/kg?d,ip) and NS group(0.25 mL,ip).After two-month treatment,all mice were killed and their bodies and spleens were weighed.Anti-dsDNA antibody in serum were detected by ELISA.The percentage of different cell subsets was checked through flow cytometry.Results(1)The spleen index、serumlevel of anti-dsDNA antibody and the percentages of CD19+、CD3+、CD3+CD4+ lymphocytes in ATO group were much lower than those in NS group of MRL/lpr mice(P0.05). Conclusion Arsenic trioxide can inhibit the activation of T and B lymphocytes,increase the percentage of NK cell and reduce the serum level of auto-antibody.And it was of no effect in the normal mice.
RESUMEN
Objective To compare the rapeutic effects of different acupoint combination applied with Rheumatoid Arthritis Cata-plasma on nuclear factor-? ? expression of CIA rats. Methods Induced arthritis rats back were endermic injected with type Ⅱ bovine collogen as animal models. There were five groups for comparison. NF-? ?, interleukin-1? (IL-1?) and tumor necrosis factor-? (TNF-?) of synovial cell clear supernatant liquid were observed. Results NF-? ?, IL-1? and TNF-? scores in therapy groups were steped down. The scores of systematic selection (distant and near coordination) of acupoints was the most close to normal group. Conclusion Rheumatoid Arthritis Cata-plasma can down-regulation the expression of nuclear factor-?? and correlated cell factor density of CIA rats synovial cell and repress positive feedback. Therapeutic effect of distant and near coordination of acupoints is better than distant selection and local selection of acupoints, probably by treating appearance and substance simultaneously to multitarget effect.