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Objective To observe the expressions of oxidized low density lipoprotein ( OxLDL ) receptor CD36 in kidney tissue of diabetic rats and in tubular cells incubated with OxLDL, and to explore the association of CD36 with the tubular injury and renal fibrosis in the process of diabetic nephropathy. Methods Diabetic rat model with hyperlipidemia was established by feeding with high sugar and fat diet and injection of low dose streptozotocin intraperitoneally. The expression of CD36 in kidney tissues was analyzed immunohistochemically. Meanwhile, the tubular sclerosis and fibrosis injury index were estimated and calculated. NRK-52E cells were stimulated with 50 mg/L OxLDL for 5, 10, 24, and 48 h, or 100 and 150 mg/L OxLDLs for 2 and 3 days. The protein expression of CD36 was detected by Western blot. Results The expression of CD36 in the renal tubulointerstitium of diabetic rats was increased comparing to that in control rats, and was localized mainly at tubular region. The renal tubular damage index(STI)ofdiabetesgroupwashigherthanthatincontrolgroup(5.54±1.5vs0.65±0.15,P<0.05). OxLDL stimulated CD36 expression in NRK-52E cells in a dose-and time-dependent manner. Conclusion The expression of CD36 was increased in renal tubular of diabetic rats, in consistent with STI. OxLDL increased CD36 expression in NRK-52E cells. These results suggest that the expression of CD36 is associated with renal tubular damages in experimental rat diabetes.
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Objective To investigate the relationship between hypothyroidism condition and renal function in diabetic nephropathy (DN) patients via measuring the hypothyroidism status of patients at different renal function stages of DN.Meanwhile,the influence of hemadialysis on thyroid function was also evaluated to pursue better treatment measures for the patients with diabetic nephropathy complicated with hypothyroidism.Observe the influence of hemadialysis in values of thyroid function,and discuss the better therapy of diabetic nephropathy with hypothyroidism.Methods FT3,FrT4,TSH3 were tested by chemiluminescence immunoassay in 125 cases of DN for January 2008 to December 2010,and SCr、BUN were tested at the same time.Then investagated the changes of thyroid function items of DN with uremia patients after hemadialysis.Analyze the data statistically.Results SCr,BUN of DN group were negatively co-related to FT3,FrT4 ( r =- 0.65,- 0.46,-0.76,- 0.56,P ＜ 0.05 ) ; But not co-related to TSH3 ( P ＞ 0.05 ).The changes of thyroid function items,namely FT3,FrT4,TSH3 of DN with uremia patients after hemadialysis was not statistically significant.Conclusion SCr,BUN were negatively correlated to FT3,FrT4,but there was no relation between SCr,BUN and TSH3.Hemadialysis therapy is not beneficial for the hypothyroidism in DN patients.