Biodegradation of nicotine in tobacco extracts for making reconstituted tobacco by strain DN2 / 生物工程学报

The purpose of the study is to use O. intermedium DN2 to degrade nicotine in tobacco extracts for making reconstituted tobacco. Firstly, we studied the effects of various factors on degradation of nicotine in the extracts by strain DN2. When we added 0.1% yeast extract into the extracts, adjusted its pH value to 7.0 by ammonia solution, inoculated 15% cultures and maintained fermentation temperature of 30 degrees C, the degradation rate of nicotine by strain DN2 was the fastest. Furthmore, under these conditions, we studied the degradation rates of nicotine in three fed batches culture which carried out in a 30-L reactor, the result showed that the average degradation rate of nicotine by strain DN2 was 140.55 mg/L/h, which was much higher than that reported in other studies. These results indicated that strain DN2 may be useful for reducing nicotine content of reconstituted tobacco.

Studies on distribution and accumulative dynamic variation of bioactive components in Eucommia ulmoides / 中草药

Object To study the effects of districts, seasonal variations and treatments in past harvest on the five main bioactive components in the barks and leaves of Eucommia ulmoides Oliv Methods The contents of them in the bark and leaves of E ulmoides were determined by RP HPLC Results The contents of (+) pinoresinol di O ? D glucopyranoside (PG), (+) syringaresinol di O ? D glucopyranoside (SG), chorgenic acid (CGA), geniposide (GP), geniposidic acid (GA) among the sample of E ulmoides from different growing districts were obvious difference Besides, the amount of different bioactive components in the same sample had no correlation Seasonal variation and different past harvest treatments had great influence on the bioactive component in E ulmoide. Based on the monthly variation of their main bioactive component, the suitable seasons for harvest of the barks and leaves of E ulmoides are April, May and July respectively, and intracellular enzymes in the fresh barks and leaves collected should be inactivated imediately before drying to avoid lossing the bioactive components In addition, between the barks and leaves of E ulmoides, the major bioactive component had great differences, but had complementarity Conclusion The districts, seasonal variatons and past harvest treatments have great effects on the contents of five main bioactive components in the barks and leaves of E ulmooides, and the barks couldn't be replaced by the leaves simply

Study on the development variation of the content of terpene lactones of Ginkgo Biliba Leaves / 中成药

Objective: To study the effectc of different growing areas and seasonal variation on the components and contents of Ginkgo Biloba terpene lactones. Methods: The contents and components of different Ginkgo Biloba terpene lactones were determined by RP-HPLC. Results: The contents of Ginkgo Biloba terpene lactones among the samples of Ginkgo Biloba Leaves of different growing areas were obviously different, the terpene lactones content of Ginkgo Biloba Leaves growing in Zaoyang county, Hubei Province, was higher. Based on the monthly variation of the contents of different Ginkgo Biloba terpene lactones, the suitable seasons for the harvest of Ginkgo Biloba Leaves were from Sep. to Oct. Conclusion: The growing areas and seasonal variation had great influence over the contents and components of Ginkgo Biloba terpene lactones.