RESUMEN
OBJECTIVE@#To explore the possible molecular mechanism of Ikaros regulation on FUT4 expression by analyzing the correlation of the functional state of Ikaros with level of FUT4 expression, so as to provide the theoretical basis for personalized treatment in children with ALL.@*METHODS@#The subtypes of Ikaros were identified by nested PCR and sequencing. The expression level of FUT4 was detected by quantitative PCR and analyzed by ΔΔCt method in the early stage of treatment, remission and relapse of ALL.@*RESULTS@#Ik1 and Ik2 were the main functional subtypes, and the dominant negative Ikaros was Ik6; the Ik6 was detected in 23 patients with ALL. It was found that 2.73% patients expressing Ik6 alone and 18.18% patients with heterozygous expression were detected. The expression of FUT4 in the newly diagnosed ALL was higher than that in the control group, and the functional Ikaros negatively correlated with the FUT4 expression(r=-0.6329).@*CONCLUSION@#Dominant negative Ikaros closely correlated with the relapse of acute lymphoblastic leukemia in children. The functional Ikaros negatively correlated with FUT4 expression. Ikaros inhibit the transcriptional activity of FUT4, that may be the molecular mechanism of Ikaros regulating the expression of FUT4.
Asunto(s)
Niño , Humanos , Enfermedad Aguda , Fucosiltransferasas , Metabolismo , Factor de Transcripción Ikaros , Metabolismo , Antígeno Lewis X , Metabolismo , Leucemia-Linfoma Linfoblástico de Células Precursoras , Isoformas de Proteínas , RecurrenciaRESUMEN
The portable gastrointestinal wireless endoscope image receiver is developed and based on TMS320C6211 DSP. It can receive and demodulate the modulated signal which is transmitted from the camera-capsule, and then output the video signal. The synchronizing signals offered by SAA7114H are made best of and are used to design the time logic circuit. The fitful video signal can be collected under the control of the time logic circuit. The circuit can automatically get rid of useless blank data and only collect effective and good-quality video signals, and storage them in CF card. In addition, the image signal can be processed and compressed by DSP, and thus the data storage space and the data- analyzing time can be saved.
Asunto(s)
Humanos , Endoscopios en Cápsulas , Endoscopía Capsular , Métodos , Sistemas de Computación , Endoscopios Gastrointestinales , Diseño de Equipo , Interpretación de Imagen Asistida por Computador , Métodos , Procesamiento de Señales Asistido por Computador , Programas InformáticosRESUMEN
To study the role of anti- C. albicans IgY and serum in protection of C. albicans infection of several animal models. To develop three animal models of C. albicans infection: a burned rat model of C. albicans infection, a mouse model of vaginal candiasis and a immunosuppression mouse model of C. albicans infection. And we compared the contribution of anti- C. albicans IgY and serum to the clearance of the C. albicans in three animal models of C. albicans infection. Anti- C. albicans IgY can protect against C. albicans infection in a burned rat model of C. albicans infection and a mouse model of vaginal candidiasis. The serum can effectively protect the mice from disseminated candidiasis in a immunosuppression mouse model. Humoral immunity component involving anti- C. albicans IgY and serum protect against C. albicans infection in a burned rat model of C. albicans infection ,a mouse model of vaginal candidiasis and a immunosuppression mouse model of C. albicans infection.