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Aim To explore the effeet of riboflavin on the establishment of pressure overload-induced heart failure model in mice by thoracic aortie constrietion (TAC ) and its preventive mechanism.Methods Eight-week-old SPF C57BL/6J mice were seleeted and divided into four groups; Sham group.Sham + ribofla¬vin group, TAC group and TAC + riboflavin group.A mouse heart failure model was constructed in the TAC group.The miee in the TAC + riboflavin group were given riboflavin by gavage one week before and eight weeks after the operation.The cardiac ultrasound inde¬xes, the changes of cardiac morphology and mitochon¬drial function indexes, the expression of apoptosis pro¬teins, ATP content, SCAD mRNA and protein expres¬sion, enzyme activity and flavin adenine dinucleotide (FAD) content in myocardial tissues were detected.Hie free fatty acid content in serum and myocardial tis¬sues were also detected.Results Compared with the sham group, the cardiac function indexes of the mice in the TAC group decreased, anrl typical heart failure occurred.Moreover, the expression of SCAD, enzyme activity, ATP and FAD content in the myocardium sig-nificantly decreased, and the free fatty acid content in myocardium and serum significantly increased.Com¬pared with the TAC group, after riboflavin treatment, the cardiac function of mice in TAC + Riboflavin group was significantly improved.In addition, ATP content, SCAD expression, enzyme activity and FAD content in myocardium all significantly increased, and free fatty acid content in myocardium and serum markedly de¬creased.Conclusions Riboflavin may improve myo-cardial energy metabolism by increasing FAD content and activating SCAD, thereby inhibiting pressure over¬load-induced heart failure in mice.
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[Abstract] Objective To determine the expression of carboxylic acid-terminal truncated hepatitis B virus X protein (Ct-HBx) in hepatocellular carcinoma (HCC) tissues and corresponding adjacent non-tumor tissues, and to explore its effect on postoperative prognosis of HCC patients. Methods Immunohistochemistry was performed to measure the expression levels of Ct-HBx in 462 HCC tissues, 263 matched adjacent tissues and 25 matched tumor thrombus tissues. The relationships between Ct-HBx expression and clinicopathological data and postoperative prognosis of HCC patients was analyzed. Results The positive rates of Ct-HBx in 263 HCC and paired adjacent tissues were 43.73% (115/263) and 11.79% (31/263), respectively. Multivariate Cox regression analysis showed that age, portal vein cancer thrombus (PVTT), preoperative aspartate aminotransferase (AST), preoperative α-fetoprotein (AFP), small daughter nodule, preoperative Barcelona Clinic Liver Cancer (BCLC) stage and postoperative antiviral treatment were independent risk factors of postoperative recurrence of HCC patients (all P0.05), and the PVTT, preoperative AFP, tumor encapsulation, preoperative BCLC stage and Ct-HBx.