Relationship between antioxidant activities and contents of active ingredients in Radix Scutellaria / 中国中药杂志

<p><b>OBJECTIVE</b>To study the relationship between the antioxidant activities and the contents of active ingredients in Radix Scutellaria.</p><p><b>METHOD</b>The antioxidant activities of extracts and active ingredients of Radix Scutellaria in NADPH-dependent lipid peroxidation in rat liver microsome were assayed.</p><p><b>RESULT</b>Extracts of Radix Scutellaria showed a 50% inhibition of lipid peroxidation in the concentration range of 2.72-18.27 mg x L(-1).</p><p><b>CONCLUSION</b>The antioxidant activities of extracts of Radix Scutellaria are not only related with the contents but also the ratio of the active ingredients.</p>

Determination of contents of the effective ingredients in root of Scutellaria baicalensis by HPLC-ECD / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC-electrochemical detection (HPLC-ECD) method for determination of contents of three effective ingredients baicalin, baicalein and wogonin in Scutellaria baicalensis simultaneously.</p><p><b>METHOD</b>70% ethanol extract of S. baicalensis was separated on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) using a mixture solution of CH3OH and H2O-THF-H3PO4(80:10:0.2) as mobile phase and detected by Waters2465 electrochemical detector at + 800 mV ( vs. ISAAC). Temperature of column was 30 degrees C.</p><p><b>RESULT AND CONCLUSION</b>The linear ranges of baicalin, baicalein and wogonin were 0.045 6-1.14 microg (r = 0.999 6), 0.013-0.325 microg (r = 1) and 0.009 5-0.047 5 microg ( r = 0.999 9), respectively. The average recoveries of baicalin, baicalein and wogonin were 97.9% (n = 5, RSD 2.3%), 97.2% (n = 5, RSD 3.3%) and 103.5% (n = 5, RSD 3.5%), respectively. The contents of three effective ingredients from 14 different germplasm resourses and 9 different growing areas of S. baicalensis were determined, the quality of S. baicalensis was evaluated.</p>