RESUMEN
Objective To approach a new method induce the differentiation of mesenchymal stems cells(MSC)into neuron-like cells.Methods We co-cultured Schwann cells with MSC to induce the differentiation of rat MSC into neuron-like cells.Immunohistochemistry was employed to observe the expression of NSE and Brdu,and Western blot to examine the expression of NeuN by in transdifferenciated MSC(Neuron-like cells).Additionally,we analyzed the differentiating rate of MSC.Results The co-culture of MSC with Schwann cells could induce the expression of NSE and NeuN.They have been differentiated into neuron-like cells.The differentiating rate of MSC was(80.51±5.65)%.Conclusion The co-culture with Schwann′s cells could differentiate rat bone marrow derived MSC into Neuron-like cells.