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Objective To explore the levels of blood N-terminal pro-brain natriuretic peptide(NT-proBNP) and homocysteine(HCY) and their clinical significance in the patients with essential hypertension(EH) complicating left ventricular hypertrophy(LVH).Methods One hundred and fifty cases of HE were divided into the EH-LVH group and simple EH group according to the results of cardiac ultrasound.One hundred individuals undergoing physical examination were selected as the control group.The levels of blood NT-proBNP and HCY were compared among three groups and the correlation analysis were analyzed with LV was performed.Results The levels of blood NT-proBNP and HCY in the EH-LVH group were obviously higher than those in the EH group and control group,the EH group was significantly higher than the control group,the difference was statistically significant(P<0.05).The levels of blood NT-proBNP and HCY had significantly positive correlation with left ventricular mass index(LVMI),while negative correlation with mitral flow velocity E/A(P<0.05).Conclusion The NT-proBNP and HCY levels can be used as the indicators for monitoring the development and progress of EH and has some predictive value for its complicating LVH.
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Connexin43 has been shown to play a pivotal role in wound healing process. Wound repair is enhanced by acute downregulation of connexin43, by increasing proliferation and migration of keratinocyte and fibroblast. Angiogenesis is also a central feature of wound repair, but little is known about the effects of connexin43 modulation on functions of endothelial cells. We used connexin43 specific small interference RNA (siRNA) to reduce the expression of connexin43 in human umbilical vein endothelial cell (HUVEC), and investigated the effects of connexin43 downregulation on intercellular communication, viability, proliferation, migration and angiogenic activity of HUVEC. Treatment of siRNA markedly reduced the expression of connexin43 by -80% in HUVEC (P < 0.05), and decreased the intercellular communication by -65% (P < 0.05). The viability, proliferation, migration and angiogenic activity of HUVEC decreased significantly (P < 0.05), compared with that of the normal cells. The results suggest that temporally downregulation of connexin43 expression at early stage of wound to inhibit the abnormal angiogenesis characterized with leaky and inflamed blood vessels, maybe a prerequisite for coordinated normal healing process.
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Objective To investigate the correlation between the anti-nuclear antibody(ANA)pattern and the specific ANA spectrum.Methods 974 cases of detected ANA results in our hospital were analyzed retrospectively.The ANA pattern was detec-ted by the indirect immunofluorescence(IIF)and the specific ANA spectrum was tested by the line immunoassay(LIA).199 cases of both simultaneous positive results by IIF and LIA were analyzed and the correlation between ANA patterns and specific ANA spectrum was analyzed.Results Among 974 cases of specimen,249 cases(25.6%)were positive by IIF and 237 cases(24.3%) were positive by LIA,the difference in the positive rate between IIF and LIA had no statistical significance(P >0.05 ).287 cases (29.5%)were positive by single IIF or LIA or both IIF and LIA,which was higher than the positive rate by single IIF or LIA (P <0.05).Among 249 cases of positive by IIF,119 cases(79.9%)were positive by LIA,among 725 cases of negative by IIF,687 (94.8%)cases were negative by LIA.The anti-Ro-52 antibody was frequently detected in the nuclear speckled pattern,and the an-ti-M2 antibody was detected more frequently in the cytoplasmic speckled pattern,anti-dsDNA antibody and anti-nucleosome anti-body were more frequently detected in the nuclear homogeneous pattern,anti-centromere antibody was more frequently detected in the centromere pattern and anti-PM-Scl antibody was more frequently detected in the nucleolus pattern.Conclusion The ANA de-tection has better correlation between IIF and LIA,but also has certain difference.Their combined detection can reduce the missed detection rate and has the important significance to the diagnosis,disease condition monitoring and prognosis judgment of autoim-mune diseases.
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Objective To compare the performance of high‐throughput ELISA and ECLIA in the determination of carcinoembry‐onic antigen (CEA) .Methods The CEA concentration of serum samples were respectively determinate by high‐throughput ELISA and ECLIA ,and the results were compared .Results Two kinds of detection methods could both accurately reflect the concentration of serum CEA .There was no significant difference between the results of two methods (P> 0 .05) .Within the linear range ,the CEA result of high‐throughput was correlate closely with that of ECLIA (r=0 .922 8 ,P<0 .01) .Conclusion High‐throughput ELISA can accurately detect the serum CEA concentration .
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Objective To establish the dose-effect curves of chromosome aberrations in human peripheral blood lymphocytes induced by different LET rays,including 60Co γ-rays,252Cf neutrons,14MeV neutrons and 12C heavy ions at low doses,respectively,in order to assess radiation-induced damages after occupational and accidental exposure.Methods Heparinized whole blood samples were irradiated with the various radiation devices and doses ranged from 0 to 1 Gy with the interval of 0.25 Gy.Conventional chromosome culture method was applied with adding colchicines at the beginning and chromosome specimens were prepared after 48 h incubation.The Metafer scanning system was used for automatical finder of chromosome metaphases.The mathematic models were fitted according to aberration data obtained.Results At the dose range of 0 - 1 Gy,the math models of dic + r were linear for 60 Co γrays and linear-quadratics for 252 Cf neutrons,14 MeV neutrons and 12 C heavy ions.The models of ace were linear-quadratic for 60Co γ-rays and 12C heavy ion beams,and linear for 252Cf and 14 MeV neutrons.The models of total aberrations were linear-quadratic for all types of radiation.Conclusions High LET rays have higher biological effects in inducing chromosome aberrations yields compared with low LET rays.Moreover,the severity of damage is 252Cf ＞ 14 MeV neutrons ＞ 12C heavy ions ＞ 60Co γ-rays in turn.Therefore,in the range of low doses,the dic + r may be a better target of radiation damage for high LET radiation.