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Objective To study the effects of polypeptide from Chlamys farreri(PCF) on PI3K/Akt and ASK1-JNK signal transduction pathway in thymocytes after ultraviolet B radiation.Methods Murine thymocytes were exposed to UVB radiation.The reactive oxygen species(ROS) level in thymocytes was detected by colormatching.The activation of Akt was investigated by western-blot.ASK1,JNK activation,mitochondria memberine potential and DNA ladder were also investigated after pretreatment with or without PI3K/Akt pathway inhibitor LY294002.Results PCF could activate Akt,inhibit the activation of the ASK1 apoptosis pathway in murine thymocyte radiated by UVB.Conclusion PCF decreased ROS level,increased Akt activity and inducing ASK1 degradation leading to the inhibition of ASK1-JNK induced apoptosis.
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Objective To observe the ultrastructural changes of Candida albicans by the effect of oyster antibiosis protein which was extracted from muscle of oyster.Methods The colonies of candida albicans exposed to oyster antibiosis protein for 24 hours,with a blank control group and the differences of their ultrastructure were studied under electron microscope.Results After affected by oyster antibiosis protein for 4h,the cell walls were lacked and had reductus with pyknotic cytoplasm.16 hours later,most cells became global shape and their cytomatrix components were lost and some vacuoles appeared in the cytoplasm.24 hours later,the cell walls depleted and the cells spitted.Conclusion oyster antibiosis protein affected commendably the function of candida albicans.