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Objective:To investigate the risk factors of postoperative recurrence of solid pseudopapillary neoplasms of the pancreas (SPN).Methods:Case-control studies on risk factors for postoperative recurrence in patients with SPN were conducted by searching in China National Knowledge Infrastructure, Wanfang Database, VIP Database, PubMed, Web of Science and Embase database from inception of these databases to November 2022. Two investigators screened the collected literatures independently according to the inclusion and exclusion criteria, extracted the data and evaluated the methodological quality, and then used Review Manager 5.4 for statistical analysis, odds ratio ( OR) was calculated with 95% confidence interval ( CI). Results:A total of 14 articles were included, including 1 409 patients with 67 cases in recurrence group and 1 342 cases in non-recurrence group. Twelve risk factors with predictive value for postoperative recurrence of SPN were extracted from the literatures. The analysis showed that the pooled OR and 95% CI of each risk factor were: gender ( OR=0.75, 95% CI: 0.35-1.59, P=0.450), age( OR=-2.08, 95% CI: -5.24-1.08, P=0.200), tumor diameter( OR=5.29, 95% CI: 4.71-5.87, P<0.001), tumor location( OR=0.56, 95% CI: 0.28-1.13, P=0.100), synchronous metastasis( OR=86.84, 95% CI: 22.64-333.05, P<0.001), lymph node metastasis ( OR=7.55, 95% CI: 2.58-22.06, P<0.001), perineural invasion ( OR=2.10, 95% CI: 0.98-4.48, P=0.060), positive margin( OR=7.00, 95% CI: 2.56-19.15, P<0.001), calcification( OR=0.49, 95% CI: 0.11-2.23, P=0.360), lymphovascular invasion( OR=11.22, 95% CI: 4.81-26.18, P<0.001), peripancreatic soft tissue invasion( OR=1.38, 95% CI: 0.48-4.00, P=0.550), capsular invasion( OR=1.72, 95% CI: 0.53-5.65, P=0.370). Conclusion:Large tumor diameter, synchronous metastasis, lymph node metastasis, positive margin, lymphovascular invasion increase the risk of recurrence of pancreatic SPN after resection, and patients with these characteristics should receive long-term follow-up.
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Objective To construct a reverse genetic platform for influenza B virus and to rescue influenza B virus.Methods Eight plasmids carrying the gene segments of B/Florida/4/2006 virus were constructed by using the bidirectional promoter vector pHW2000.293T cells were co-cultured with MadinDarby canine kidney (MDCK) cells and then transfected with the eight plasmids.The supernatants of cell culture and cell debris were collected after transfection and then injected into embryonated chicken eggs and MDCK cells for rescuing the influenza B virus strains.Results This reverse genetic system could be used for the preparation of reassortant influenza B virus strains.The titers of hemagglutination units of the rescued virus achieved 128-256/50μl.Most of the reassortant virus particles were spherical under electron microscope.Conclusion The pHW2000 reverse genetic system could be used for the rescue of influenza B virus.Moreover,it could also be used for the construction of influenza B virus with specific mutations for further in vestigation on the characteristics of influenza B virus and the construction of vaccine strain.