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Objective To explore the titre of serum anti-phospholipase A2 receptor antibodies (anti-PLA2 R antibodies) detected by the enzyme-linked immunosorbent assay (ELISA) to provide a more specific serological index for clinical diagnosis and disease judgment of membranous nephropathy (MN).Methods Thirty-four cases of MN confirmed by kidney biopsy ,32 inpatients with autoimmune diseases ,nephrotic syndrome and renal insufficiency in the nephrology department of our hospital and 12 persons un-dergoing physical examination were selected.The serum was collected for detecting anti-PLA2 R antibodies level.Then its diagnostic performance for diagnosing MM was analyzed by combining with serum TP ,ALB ,IgG ,IgA ,IgM ,C3 and C4 indicators.Results The medians of anti-PLA2 R antibodies titres in the MN group ,disease control group and healthy control group were 22.1 ,2.0 ,2.0 RU/mL respectively ,which had statistical difference between the MN group and other two groups (P0.05).Conclusion Anti-PLA2 R antibodies have higher specificity for the diagnosis of MN and can serve as the necessary and specific serologic detection indicator in the patients unable to conduct renal biopsy.Quantitative detection helps to condition judgment.
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Objective To understand the differences of competency assessment of residents from the hospital director of clinical departments and resident physicians and to explore residency training mode for future ability training of the residents .Methods Study was performed in a third-grade class-A hospital in Beijing to understand the difference of capacity ,creativity evaluation be-tween the directors of clinical department and resident physicians through a questionnaire survey and statistical analysis .Results Results showed no statistically significant differences between the directors of clinical department and resident physicians in com-puter application ,and the remaining capacities were lower in the directors of clinical department than in the resident physicians .Be-sides ,the resident physicians hold that the residents were poor in research capacity ,creativity and legal awareness .Conclusion The study prompts us to strengthen the clinical expertise and skills training of residency ,also we should pay attention to the training of comprehensive ability .
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Objective To investigate the effects and the mechanisms of tissue factor pathway inhibitor（TFPI）on no-reflow（NR）after acute myocardial infarction（AMI）and reperfusion in rabbits.Methods Rabbits were randomly divided into sham operation group,saline control group and TFPI group.The model of NR after AMI and reperfusion was induced by ligating coronary artery for 60 minutes and reperfusion for 90 minutes.The expressions of interleukin-6（IL-6）,tumor necrosis factor alpha（TNF-α）and tissue factor（TF）were determined by immunohistochemistry.No-reflow area（NRA）was evaluated by thioflavine S staining.The ligation area（LA）and necrosis area（NA） were evaluated by Evans blue and triphenyltetra zolium chloride（TTC）staining.Results After 90 minutes of reperfusion,the levels of IL6 and TF in saline control group were all singnificantly higher than those of TFPI and sham operation group（P 0.05）.There was no statistical difference in the expression of TNF-α（P 0.05）.There was no statistical difference of LA between saline control group and TFPI group（P 0.05）,while NRA and NA were markedly reduced in TFPI group compared with saline control group（P 0.05,P 0.01）.Conclusion No-reflow happened after ligating coronary artery for 60 minutes and reperfusion for 90 minutes in rabbits.TFPI could reduce NRA and NA,and the thrombosis and inflammation might be involved in the mechanisms.
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BACKGROUND: Stem cell transplantation can significantly improve heart function foUowing myocardial infarction. This is correlated with the differentiation of stem cells into cardiomyocytes and promotion effect on angiogenesis. Paracrine and ventricular reconstruction inhibition (especially extracallular collagen reconstruction) have important effects on improving heart function.OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on coUagen remodeling after acute myocardial infarction in rabbits.DESIGN, TIME AND SETTING: The randomized controlled animal study was performed at the Laboratory of Acupuncture and Electrophysiology of Liaoning University of Traditional Chinese Medicine from June to August 2007.MATERIALS: A total of 57 healthy Japanese rabbits were purchased from Experimental Animal Center, Uaoning University of Traditional Chinese Medicine.METHODS: BMSCs were acquired from the bone marrow of two rabbits, and marked with BrdU before transplantation. Ten rabbits served as a normal group. Forty-five rabbits were used to establish the left ventricular infarct by ligation of the left coronary artery. Thirty success models of myocardial infarction were randomly divided into 3 groups (n=10)" model, saline and call transplantation groups. Following 7 days of myocardial infarction, rabbit models in the cell transplantation group were injected in the ear vein with 1 mL of BMSCs (2x106 cells). Rabbits in the saline group were infused with 1 mL of saline. The culture was performed for 5 weeks.MAIN OUTCOME MEASURES: Fibrous structure of myocardial stroma was observed, and collagen volume fraction was measured by Masson Trichrome staining. The ratio of type Ⅰ and Ⅲ collagen was determined by immunohistochemistry.RESULTS: BrdU-positive BMSCs could be seen in the cell transplantation group. After myocardial infarction, a few collagen fibers was confluent in or surrounding the infarct area, arranged orderly in the cell transplantation group. Collagen fiber plaque-shaped confluence was significant, and arranged disorderly in the model and saline groups. At 5 weeks following myocardial infarction, compared with the normal group, collagen volume fraction was significantly decreased in and surrounding the infarct region (P < 0.05), and the ratio of type Ⅰ and Ⅲ collagen was increased significantly in the model group (P < 0.05).Compared with the model group, collagen volume fraction and the ratio of type Ⅰ and Ⅲ collagen were significantly decreased (P< 0.05).CONCLUSION: BMSCs could survive in infarct heart. BMSCs transplantation could reduce collage volume and improve collage ratio and had beneficial effects on collage remodeling processes after acute myocardial infarction.