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Objective Using the previously established mesenchymal stem cells strain derived from human fetal umbilical cord blood (FUCB-MSCs) to culture then label enhanced green fluorescent protein (EGFP) , and to observe skin repair effects of FUCB-MSCs by GFP tracing after exogenous FUCB-MSCs transplantation on to scald wound models of SCID mice.Methods FUCB-MSCs were labeled GFP by transfection with the recombinant retrovirus containing EGFP gen;The established SCID scald mice model were randomLy divided into 3groups, low dose group, high dose group and control group, 6rats each group, 2wounds each mouse, 12wounds in total, then were tail intravenous injected into 0.2mL 1×106, 0.2mL 2×106 GFP-FUCB-MSCs cells, and same volume of medium respectively.On 9days after transplantation, the sections from scald wound area were observed the expression of GFP under the fluorescence microscope and the others were analyzed by the bright-field microscopy after HE staining, and the area of wound surface and the number of wound cells were compared simultaneously.Results After 48h, expression of EGFP in FUCB-MSCs can be seen under the fluorescence microscope, positive rate of GFP was>80％, and after 6weeks GFP expression is still stable, besides, the positive expression of human GFP can be observed after transplantation and there were no fluorescence decay in transplantation after 3weeks.Compared with the control group, there was a significant difference in wound area and wound cell number in the low and high-dose group (P<0.05) .ConclusionGFP can be used as a tracking marker to label FUCB-MSCs during transplantation treatment.It indicates that exogenous FUCB-MSCs can migrate to the scalded wounds via blood circulation system and continuously participate in the repair through SCID mouse.
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Objective To investigate the therapeutic effect of stage treatment of severe complicated injuries in palmar forearm and wrist.Methods From March 2008 to Novemler 2011, eleven cases with skin lost combined with fracture and lost of flexor tendon, ulnar radial arteries, median and ulnar nerves,and some of them had fracture of ulnar and radius were treated in the first stage with vacuum sealing drainage after emergency debridement and tissue repair. Secondary microsurgical repair was performed after the VSD was removed. The lost of median and ulnar nerves was repaired with transplantation of sural nerve,and the wound was repaired with free flaps and complex tissue flaps. Results Eleven cases were treated with vacuum sealing drainage for 7 to 10 days.Infections were under control,wound areas reduced and most wound areas were covered by granulation tissues.Flaps and skin grafts were survived.After postoperative follow-up for 3 to 36 months (average 13 months),three cases was received flexor tendolysis.As result,the blood circulation of hand was good, the sensor of injured nerve was higher than S3, and there was a good functional rehabilitation of hand muscles. Conclusion Using vacuum sealing drainage after emergency debridement and tissue repair, there was a good repairing effect of secondary repair with flaps and skin grafts for severe complicated tissue defect in palmar forearm and wrist,and it is an effective choice of operation.
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Objective To summarize the clinical experiences in microsurgical treatment of severe children hand injuries caused by firecrackers. Methods 26 cases of children hand injuries caused by a firecracker blast were treated with microsurgical procedures. An emergency operation was done for 23 cases, and a delayed repair was done for 3 cases. Operative procedures differed from case to case, according to the injury type. Results All flaps and reconstructed thumbs survived completely by first intention. Of the 9 cases with 11 fingers replanted, 7cases with 9 fingers survived. The follow up for over two years showed that satisfactory restoration of hand function and shape was achieved and the replanted fingers also showed satisfactory restoration of holding and finger to palm and finger to finger functions. Conclusion Severe children hand injuries caused by a firecracker blast must be treated as soon as possible with microsurgical procedures. Preoperative planning of a proper repair or reconstruction approach is also of great importance.
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OBJECTIVE:To measure the concentrations of levofloxacin in aqueous humor after a single eye dripping of drug and calculate the pharmacokinetic parameters.METHODS:32 rabbits were divided into 8 groups.The rabbit eyeballs were enucleated to make a collection of aqueous humor at 0.125,0.25,0.5,0.75,1.0,2.0,3.0 and 4.0 hours after dripping.All measurements were performed with HPLC.RESULTS:In aqueous humor,Tmax:0.940h,Cmax:0.592?g/ml,T1/2(Ka):0.479h,T1/2 (Ke)0.919h.By using 3p87 pharmacokinetic program,the concentration-time curves were fitted as one-compartment op_en model.CONCLUSION:All the concentrations of levofloxacin at different times after dripping in aqueous humor exceeded the minimum inhibitory concentration for 90%(MIC90)of most bacterial pathogens.These results show that levofloxacin lactate eye-drops may be an effective antimicrobial agent for the treatment and prevention of intraocular infections as well as other ocular infections.
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OBJECTIVE:To develop a preparation and quality control method for sinomenine gel.METHODS:Sinomenine gel was prepared with carpol971as base.TLC and UV were employed for identification of sinomenine.Sinomenine was deter?mined by RP-HPLC.RESULTS:The TLC identification was highly specific and the spots were clear.Recovery was99.6%?1.3%.CONCLUSION:This paper provides a rapid,convenient and accurate method which is suitable for preparation and quality control for sinomenine gel.
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The prescription composition rules and special features of compound anti-influenza preparationS made from Chinese and western medicines are explored in this paper. The problems in the prescription composition rules are also discussed.