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National Journal of Andrology ; (12): 103-109, 2017.
Artículo en Chino | WPRIM | ID: wpr-812802

RESUMEN

Objective@#To investigate the expression characteristic of the Daxx gene in the mouse testis and its role in spermatogenesis.@*METHODS@#Real-time PCR, Western blot and immunofluorescence were used in examining the expression characteristics of DAXX in the testis tissue from wild-type, Sertoli cell-specific androgen receptor knockout (SCARKO) and androgen receptor knockout (ARKO) mice at different postnatal weeks .@*RESULTS@#The Daxx gene was highly expressed in the testis tissue and mainly in the nuclei of the wild-type mice at 4 postnatal weeks. Compared with the wild-type, the ARKO mice showed a markedly decreased expression of DAXX (0.299±0.026), which displayed a polar distribution in the spermatogenic cells (0.853±0.058) and exhibited no significant difference in the SCARKO mice (1.000±0.015).@*CONCLUSIONS@#The Daxx gene expression is the highest in the middle-stage development of the mouse testis, significantly decreased in ARKO mice as compared with the wild-type, and its location influenced by specific AR knockout in Sertoli cells. DAXX may be involved in the regulation of spermatogenesis in mice.


Asunto(s)
Animales , Masculino , Ratones , Proteínas Portadoras , Genética , Metabolismo , Núcleo Celular , Genética , Metabolismo , Expresión Génica , Péptidos y Proteínas de Señalización Intracelular , Genética , Metabolismo , Ratones Noqueados , Chaperonas Moleculares , Proteínas Nucleares , Genética , Metabolismo , Receptores Androgénicos , Genética , Células de Sertoli , Espermatogénesis , Genética , Testículo , Metabolismo
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