Value of Minimum Apparent Diffusion Coefficient in Peritumoral Edema in the Differential Diagnosis between Primary Central Nervous System Lymphoma and Glioblastoma / 中国医学科学院学报

Objective To evaluate the role of minimum apparent diffusion coefficient(MinADC) values in peritumoral edema based on magnetic resonance diffusion weighted imaging in the differential diagnosis between primary central nervous system lymphoma(PCNSL) and glioblastoma(GBM).Methods ADC values in peritumoral edema were measured in 16 patients with PCNSL(diffuse large B cell lymphoma) and 31 patients with GBM(WHO grade 4) confirmed by pathology.Regions of interests were manually drawn on ADC maps on peritumoral edema regions to obtain the MinADC value.Independent samples t-test and receiver operating characteristic analysis were performed for statistical analysis.Results The MinADC value [(1.20-1.45)×10 mm/s,mean(1.35±0.68)×10 mm/s] in PCNSL was significantly higher than that in GBM [(0.95-1.31)×10 mm/s,mean(1.12±0.09)×10 mm/s](t=9.977,P=0.000).The area under the receiver operating characteristic curve was 0.986,and the cutoff value of MinADC was 1.245×10 mm/s for the differentiation between PCNSL and GBM,with the best combination of sensitivity(94.1%) and specificity(94.1%).Conclusion MinADC value can be a simple and effective measure for the differential diagnosis between PCNSL and GBM.

Evaluation for impacts of gut wall metabolism of multiple components in Chuanxiong Rhizoma during intestinal absorption with S9 incubation / 中国中药杂志

The information of drug deposition in the intestine is required in the study for the drug absorption in biopharmaceutics classification system (BCS). To illustrate the impacts of gut wall metabolism on the absorption, metabolism of multiple components in Chuanxiong Rhizoma in gut wall was tested by rat S9 incubation in vitro. The chemical fingerprint technology was used in this study to simultaneously detect multiple components in Chuanxiong, and peak areas before and after S9 incubation were compared. The results showed that senkyunolide I and several constituents were metabolized by gut wall, and one new metabolite was founded. However, ferulic acid and other compounds remained unchanged after incubation. Therefore, the subsequent intestinal permeability of multiple components in Chuanxiong that were not metabolized in the intestine was suggested to be detected directly by in situ single-pass intestinal perfusion (SPIP).Nonetheless, the intestinal permeability of the constituents that were metabolized in the intestine shall be explored by appropriate approaches.

Suppression of tumor growth in renal cancer treatment with tumor vaccination after haploidentical bone marrow cell reconstitution / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate whether whole tumor cell vaccination strategies in combination with bone marrow transplantation (BMT) can stimulate graft-versus-tumor effect (GVT).</p><p><b>METHODS</b>Twenty-six BALB/c mice were randomly divided into 3 groups: BMT group (group A, n = 10), BMT + vaccination group (group B, n = 10), control group (group C, n = 6). (BALB/c × C57BL/6) F1 mice [CB6F1, H-2K(b/d)] were used as donors. BALB/c mice of group C were only inoculated with Renca cell (2.6 × 10(6)). Mice of group A and B were conditioned with 8 Gy irradiation, followed by infusion by bone marrow cell of CB6F1 mice on day 1, then inoculated with Renca cell (2.6 × 10(6)) on day 8. All mice of group B were immunized subcutaneous on the back with 5 × 10(5) irradiated Renca tumor cells on day 9 and day 16. All mice of group C were inoculated with Renca cell (2.6 × 10(6)) on day 8. In group A and B, all mice were analyzed by fluorescence activated cell sorter (FACS) on day 14, and 28 day after BMT. Mice were killed on day 32 after inoculation with tumor cell and collected blood sample. All tumors were taken out to be weighed and then fixed in 10% buffered formalin, embedded in paraffin, and cut into 5 µm slices. The slices were stained with HE and examined by TdT mediated-dUTP nick end labeling (TUNEL). Liver, skin, intestine, and spleen were biopsied for histopathological examination.</p><p><b>RESULTS</b>The results of chimera showed that engraftments of group A, B were full donor chimerism, and the chimerism of those remained above 90% and preserved even after 28 days. The tumor weight, tumor volume increment in the group B was lower than group A and C (P < 0.05). The tumor suppressing rates of the group A and B were 54%, 60% respectively. The area ratio of tumor necrosis and apoptosis index (AI) of the tumor in the group B were higher than group A and C (P < 0.05). Graft-versus-host disease was not observed in each group.</p><p><b>CONCLUSION</b>The mechanism of GVT after haploidentical allogeneic bone marrow transplantation with tumor vaccination may be the promotion of tumor necrosis and apoptosis.</p>

Time course of ubiquitin and rat component 3 of proteasome expressions and association with common carotid artery intimal proliferation after balloon injury in rats / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the dynamic ubiquitin and rat component 3 of proteasome (RC3) change and association with intimal proliferation in the common carotid artery of rats after balloon injury.</p><p><b>METHODS</b>Male SD rats were randomly divided into two groups: sham operation group (n = 8), ballon injury group at various time points (1, 4, 7, 14, 21, 28-days post injury, n = 8 each). The balloon injury model was established by deendothelializing the rat common carotid aortic artery by inflated 2F balloon catheter. HE staining was used to observe the ratio of intima-media (I/M) thickness of the injured arteries under optical microscopy. The expression levels of ubiquitin and RC3 mRNA were detected with RT-PCR. The expression level of ubiquitin protein was measured by immunohistochemistry method.</p><p><b>RESULTS</b>The maximal intimal proliferation and peak intima-media ratio (2.31 +/- 0.43 vs. control 0.02 +/- 0.005, P < 0.01) were seen at 28th day post injury. The peak expression of ubiquitin was observed at the 7th day post injury (1.33 +/- 0.26 vs. control 0.21 +/- 0.04, P < 0.01) and peak RC3 expression was found at the 14th day post injury (1.35 +/- 0.26 vs. control 0.31 +/- 0.06, P < 0.01). The protein expression of ubiquitin was also seen at the 14th day post injury (21.53 +/- 4.09 vs. control 4.21 +/- 0.78, P < 0.01). The expression of ubiquitin protein was positively correlated with intimal proliferation(r = 0.827, P < 0.05).</p><p><b>CONCLUSION</b>The upregulated ubiquitin and RC3 expressions after balloon injury might play an important role in intimal proliferation in this model.</p>

Effect of Simvastatin on the Expression of Ubiquitin and Rat Component 3 of Proteasome in the Common Carotid Artery of Rats After Balloon Injury / 中华高血压杂志

Background The ubiquitin-proteasome pathway(UPP)involves 80%-90% degradation of all in- tracellular proteins.Both ubiquitin and rat component 3 of proteasome(RC3)are hence considered to be central me- diators for cell biology.Objective To evaluate the effect of simvastatin on neointimal hyperplasia and the expres- sion of ubiquitin and rat component 3 of proteasome(RC3)after balloon injury in carotid artery.Methods Thirty- two male SD rats were randomly to receive,low dose(0.4 mg/ng,n=8),or moderate(4 mg/ng,n=8),or high- dose(40 mg/ng,n=8)of simvastatin treatment for 28 days.Common carotid aortic artery was injuried rat ballon. The ratio of intima-media(I/M)thickness was determined.The expression level of ubiquitin and RC3 mRNA were assessed by RT-PCR.The expression level of ubiquitin protein was examined with immunohistochemistry. Results Simvastatin inhibited the expression of ubiquitin and RC3 mRNA and ubiquitin protein in dose dependent manner.The intima-media ratio(-50.2 %)and the expression of ubiquitin(-60.3 %)and RC3 mRNA and ubiq- uitin protein(-60.5 %)was reduced by the high dose simvastatin [40 mg/(kg?d)](P