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Journal of Central South University(Medical Sciences) ; (12): 167-170, 2005.
Artículo en Chino | WPRIM | ID: wpr-813412

RESUMEN

OBJECTIVE@#To clone and characterize new genes of Schistosoma japonicum, Sj, and to provide efficient vaccine candidates.@*METHODS@#Sj adult cDNA library was screened with rabbit sera raised against male worm soluble antigen. The inserted cDNA fragments from the positively selected clones were amplified with PCR and further sequenced, as well as characterized through internet NCBI GenBank software.@*RESULTS@#Eleven positive clones were obtained and two were verified by GenBank as new, including a novel gene designated as Sj-P8 (GenBank accession No. AF517843) and a new partial cDNA of Sj myosin (GenBank accession No. AY770506). The two new genes encoded a transmembrane protein of 75 amino acids and a myosin protein fragment of 212 amino acids respectively.@*CONCLUSION@#The newly obtained genes may provide useful information for the research on Sj vaccine.


Asunto(s)
Animales , Masculino , Conejos , Secuencia de Aminoácidos , Antígenos Helmínticos , Genética , Alergia e Inmunología , Secuencia de Bases , Clonación Molecular , ADN Complementario , Genética , ADN de Helmintos , Genética , Biblioteca de Genes , Genes de Helminto , Genética , Datos de Secuencia Molecular , Schistosoma japonicum , Genética , Esquistosomiasis Japónica , Vacunas Sintéticas
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