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1.
Chinese Journal of Medical Education Research ; (12)2006.
Artículo en Chino | WPRIM | ID: wpr-686772

RESUMEN

This paper investigates the main problems existing in research-type key lab construction in higher schools.Based on the investigation,countermeasures are proposed also.

2.
Journal of Forensic Medicine ; (6): 155-159, 2004.
Artículo en Chino | WPRIM | ID: wpr-983037

RESUMEN

OBJECTIVE@#For the purpose of solving a problems of DNA testing of burned bones.@*METHODS@#We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purification using the DNA IQ System.@*RESULTS@#The methods were found to be effective in removing the PCR inhibitors from the burned bone. Then the extracted DNA was successfully genotyped by using the florescence labeling STR multiplex method.@*CONCLUSION@#The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, precluding the utilization of traditional forensic DNA identification techniques.


Asunto(s)
Femenino , Humanos , Masculino , Huesos/química , Quemaduras/metabolismo , Compuestos de Cetrimonio , ADN/aislamiento & purificación , Dermatoglifia del ADN/métodos , Medicina Legal/métodos , Reacción en Cadena de la Polimerasa/métodos , Sensibilidad y Especificidad , Secuencias Repetidas en Tándem , Factores de Tiempo
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