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Objective To evaluate the validity of percutaneous radiofrequency ablation(RFA)combined with percutaneous ethanol injection(PEI) with different style in rabbit liver in vivo.Methods Twenty-four New Zealand white rabbits were included in this study and divided into four groups.Group A:RFA before PEI(n=6),RFA (1 cm mono-electrode,maintain 3 minutes RFA) before PEI 1.5 ml; Group B:PEI before RFA (n=6),PEI 1.5 ml before RFA (1 cm mono-electrode,maintain 3 minutes RFA); Group C:RFA (1 cm mono-electrode,maintain 3 minutes RFA) only (n =6);Group D:PEI (1.5 ml) only(n=6).To analyze the resistance,current and energy requirement per unit of each group including RFA.To observe the size,shape,isoperimetric ratio and volume of coagulated necrosis of each group by enhanced CT.Results The longest diameter and the shortest diameter of group B[respectirely,(24.1±4.4) mmand (21.45±4.0) mm] were significantly larger than group C [respectirely,(12.4 ± 1.6) mm and (11.1 ± 1.4) mm] and group D[respectirely,(7.7 ± 2.3) mm and (5.1 ± 1.5) mm] (P＜0.01).The height diameter and volume of coagulated necrosis of group B [respectirely,(20.3± 4.9) mm3 and (5879 ± 2607) mm3] were significantly larger than the other 3 groups [(14.8± 2.7) mm3 and (3130±1250) mm3,(10.7±1.6) mm3 and (767±173) mm3,(6.7± 1.0) mm3 and (146±83) mm3] in A,C,and a group (P＜0.01).Isoperimetric ratios of ablation zone in group B was the most highest.There were no statistically significant between each group (P＞0.05).The resistance of group B were significantly larger than group A and group C (P＜0.01).The current of group B were significantly lower than group A and group C (P＜0.05).The energy requirement per unit of group A and group B were significantly lower than group C (P＜0.01).Conclusion The volume of coagulated necrosis of group PEI-RFA was significantly larger than the other 3 groups.The energy requirement per unit of group PEI-RFA were the lowest in each group.The isoperimetric ratio of group PEI-RFA was the most highest.
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OBJECTIVE@#To examine the expression of mRNA of Oxford 40(OX40) and Oxford 40 ligand(OX40L) in the sciatic nerve, spleen, peripheral blood mononuclear cells and lymph nodes of experimental allegic neuritis (EAN).@*METHODS@#Thirty-six Lewis rats were randomly assigned into an EAN group and a CFA group. The rats were sacrificed on 9th, 17th, and 26th day after immunization. OX40 and OX40L mRNA was detected by reverse transcription polymerase chain reaction in the spleen, sciatic nerves, peripheral blood mononuclear cells and lymphonodes.@*RESULTS@#The peak of clinical course came on 17th day after the immunization in EAN. The mRNA expression of OX40/OX40L was higher on 8th day and 17th day than that on 26th day after the immunization (P<0.05). There was significant difference between the EAN group and the CFA group at the 3 time points (P<0.05); rats in the CFA group didn't have any clinical manifestations. The mRNA expression of OX40 and OX40L in the EAN group raised in the sciatic nerves and lymph nodes at the above 3 time points (P<0.05). Weak expression was seen in the peripheral blood mononuclear cells.@*CONCLUSION@#OX40 and OX40L may play a role in the pathogenesis of experimental allegic neuritis.
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Animales , Femenino , Ratas , Glicoproteínas de Membrana , Genética , Metabolismo , Neuritis Autoinmune Experimental , Genética , Metabolismo , ARN Mensajero , Genética , Metabolismo , Distribución Aleatoria , Ratas Endogámicas Lew , Receptores OX40 , Genética , Metabolismo , Nervio Ciático , Metabolismo , Factores de Necrosis Tumoral , Genética , MetabolismoRESUMEN
Objective To study the expression of mRNA of OX40 and OX40L in the sciatic nerve,spleen,peripheral blood mononuclear cells and lymph nodes of EAN under the influence of Rho-kinase inhibitor.Methods All 54 female Lewis rats were divided into 3 groups:the EAN group,the EAN+ Rho-kinase inhibitor group and the complete Freund's adjuvant(CFA)group.The rats were sacrificed at 9,17 and 26 days after immunized.Ox40 and OX40L mRNA were detected by RT-PCR which came from spleens,sciatic nerves,peripheral blood mononuclear cells and lymphonodes.Results In EAN+ Rho-kinase inhibitor group,the mRNA expression of OX40 were 0.266±0.031,0.298±0.024 and 0.113±0.018 at 9.17 and 26 days in the sciatic nerve,the expression were 0.453±0.030,0.496±0.100 and 0.220±0.016 in the lymph nodes.The mRNA expression of OX40L were 0.247±0.018.0.298±0.026 and 0.165±0.013 in the sciatic nerve,the expression were 0.283±0.027,0.306±0.011 and 0.161±0.012 in the lymph nodes.The mRNA expression of OX40 and OX40L in EAN+Rho-kinase inhibitor group was lower than EAN group at the three time points(t=2.24-4.89,P＜0.05),and the demyelination and inflammation cells infiltrating were ameliorated in spinal nerve.CFA group didn't show any clinical manifestation.Conclusion Rho-kinase inhibitor may ameliorate tlIe development of EAN through inhabiting the OX40 and OX40L activation.
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Objective To study the effect of Rho-kinase inhibitor on experimental allergic neuritis. Methods 54 female Lewis rats were divided into three groups; EAN group, EAN + Rho-kinase inhibitor group, and CFA group. The rats were sacrificed on the 9th day, 17th day, and 26th day after immunized. The changes of weight, EAN incidence, and mean day of onset, mean maximum clinical score, and histopa-thology were observed. Results The clinical course in EAN group reached peak on the 17th day. Compared with EAN group, the weights of Rho - kinase inhibitor group were increased, while EAN incidence, mean day of onset delay, and the clinical scores in Rho-kinase inhibitor group were significantly decreased, ( P < 0.01) , and the demyelization and inflammation cells infiltrating was ameliorated in spinal nerve. CFA group didnt show any clinical manifestation. Conclusions Rho - kinase inhibitor may ameliorate the development of EAN through inhabiting the Rho/ROK signal pathway.