Changes of elastic fibers in middle cerebral artery of different aging rats / 中国组织工程研究

BACKGROUND: Changes of elastic fibers in middle cerebral artery(MCA)is close related withthe aged cerebrovascular disease.OBJECTIVE: To observe the changes of elastic fibers of MCA in different aging rats.DESIGN: A descriptive and controlled study based on experimental animals.SETTING: Department of anatomy and central laboratory in a university.MATERIALS: Totally 36 healthy Wistar rats with either gender, weighing 200 - 280 g, were selected from the Animal Institute of the third medical military university of Chongqing[certification SCXX (army) 2002-007].INTERVENTIONS: Changes of elastic fibers of MCA of different aging rats were observed with light microscope, transmission electron microscope and image analysis system. MAIN OUTCOME MEASURES: ①) Major outcome: changes of elastic lamella in MCA of different aging rats; ②) Secondary outcome: ultramicrostructural changes of internal lamella under the transmission electron microscope. RESULTS: With the increase of age, the folded extent and quantity of internal elastic lamella were decreased, and the content of elastic fibers were also decreased significantly( P ＜ 0.01 ). However, the ratio of collagen fibers to elastic fibers was increased significantly( P ＜ 0.01 ) . In the aging group above 24 months, the internal elastic lamina thinned, delaminated and disrupted, and the lipid deposited in it. Endothelial cells and smooth muscle cells passed through the internal elastic lamina. CONCLUSION: Changes of elastic fibers may be related with the increased susceptibility to the cerebrovascular disease in aged people.

Overexpression of TGF beta 1 increases elastin expression and adhesion of smooth muscle cells / 生物医学工程学杂志

This study was conducted to examine the effectiveness of a gene transfer of human TGF beta 1 gene into smooth muscle cells and whether the TGF beta 1 can increase elastin expression of smooth muscle cells. With the help of DOTAP, smooth muscle cells were transfected with pMAMneoTGF beta 1. The positive cell clones were selected with G418. The stable transfection and expression of TGF beta 1 in the smooth muscle cells were determined by immunofluorescence analysis. The expression of elastin in the transfected and untransfected cells were determined by in situ hybridization. The adhesion force between smooth muscle cells and matrix was detected by micropipette system. The results showed abundant TGF beta 1 stable expression in smooth muscle cells. TGF beta 1 gene can increase two-three times elastin expression and increase the adhesion between smooth muscle cells and matrix. TGF beta 1 can be used in vascular tissue engineering to increase smooth muscle cells adhesion.

Recent advances in artrial elastin / 中国病理生理杂志

A Review The elastin has been investigated since the 1950 s, and it has been found that elastin relates to the vascular diseases in recent years. In this review, the elastin gene structure, distribution and its role in the pathogenesis of vascular diseases and aging are summarized.

Thin sectional anatomy of the cardiac septum and surrounding structure / 第三军医大学学报

Objective To study the sectional anatomy of the cardiac septum to provide the reference for clinical imageology and surgery. Methods Sixteen normal adult hearts without organic lesions were verified macroscopically. After vascular perfusion, the specimens were embedded with gel, fixed with 5% formalin, and cryopreserved for a week, and then were sectioned with the Digital Sectioner. Results A total of 1 608 slices (thickness: 0.2 mm) of the heart were obtained. Cardiac septum and the surrounding structures were shown clearly. The demarcation of connective and muscular tissues was clear. The diameters of the cardiac apex, fossa ovalis, brawny intraventricular septum, left ventricle, right ventricle, left and right fibrous trigone were measured with Photoshop6.0. Conclusion The clear images can display tiny structures that could be measured, which could provide anatomical references clinical imageology and surgery.

The Macro-Micro-Anatomy of the Blood Vessels in the Medial Genicular Cutaneous and Myocutaneous Flap / 第三军医大学学报

The descending genicular vessels and their branches were observed on 50 adult lower extremities.The saphenous branch supplies the skin on the medial genicular region.It has a larger caliber and a longer pedicle.The area distributed by this branch is 60.96?35.01cm2.The saphenous branch also gives out branches to the lower one third of the satorius muscle.The descending genicular vessels and it's articular branch in 88% cadavers give out the branches to the vastus medialis muscle.In addition,the great saphenous vein and the saphenous nerve were observed.The design for the medial genicular cutaneous flap and myocutaneous flap were discussed.

MORPHOLOGICAL OBSERVATION AND QUANTITATIVE ANALYSIS OF STRUCTURAL COMPONENTS OF HUMAN UPPER LIMBARTERIES ON SECTIONS / 解剖学报

The sectional morphology of the human brachial artery, ulnar artery, radial artery, palmar digital artery of the thumb and the middle finger were observed under microscopy. Excepting the brachial artery, the intima of the other 4 arteries are thick, especially the ulnar artery. The histological variations must be noticed during the artery is anastomosed. The percentage of the adventitia of the whole wall thickness decreases and the elastic fibers become looser gradually in accordance with the size of the arteries. The relative contents of elastin, collagen and smooth muscle of the above 5 arteries were measured by microspectrophotometer. The content of elastin of these arteries decreases(P

THE MACRO-MICRO-ANATOMY OF THE VESSELS OF THE SKIN FLAP IN THE FRONTO-PARIETO-TEMPORAL REGION / 解剖学报

The vessels of the skin flap in the fronto-parieto-temporal region were studied by way of injecting 10% ethyl acetate dye into the artery of 25 Chinese adult cadavers.The main arterial supply in this region is the superficial temporal artery. In addition,the posterior auricular,supraorbital and supratrochtear arteries distribute over this region as well.The superficial temporal artery is the largest scalp artery.The area distributed by this artery amounts to 57.38?6.05% of the whole scalp.The frontal and parietal branches are the terminal branches of the superficial temporal artery.The former is a constant branch in our cases.It is a dominant vessel of which both the distributing area and the caliber are larger than the parietal branch.The superficial temporal vein is mainly formed by the union of a frontal and a parietal venous branches,which approximately accompany the corresponding branches of the artery. The arteries in the fronto-parieto-temporal region form rich anastomoses at the median area of the vertex.The large anastomoses channels were counted in this study.These channels have anatomically provided for the designing of a supe(?)long flap which run across the median zone.The forehead flap with the frontal branch as the pedicle artery and the scalp flap with the parietal branch as the pedicle artery were discussed.