Simultaneous Determination of 8 Components in Lyophilized Product of Chaihu Shugan San Decoction Based on UPLC / 中国药房

ABSTRACT OBJECTIVE：To establish a method for simultaneous determination of 8 components in the lyophilized product of Chaihu shugan san decoction. METHODS：UPLC method was adopted to determine the contents of albiflorin，paeoniflorin，ferulic acid，naringin，hesperidin，benzoyl paeoniflorin，glycyrrhizic acid and α-cyperone in 6 batches of lyophilized product of Chaihu shugan san decoction. The determination was performed on Phenomenex column with mobile phase consisted of 0.1％ formic acid water-acetonitrile at the flow rate of 1 mL/min，the sample volume was 10 μL. The detection wavelength was set at 250 nm，and column temperature was 30 ℃. RESULTS：The linear range of albiflorin，paeoniflorin，ferulic acid，naringin，hesperidin，benzoyl paeoniflorin，glycyrrhizic acid and α-cyperone were 3.606-8.414，23.988-55.972，1.218-2.842，35.964-83.916，12.009-28.021， 1.194-2.786，3.609-8.421，5.294-12.352 μg/mL，respectively（r＝0.999 5-0.999 9）. The limits of quantitation were 0.206，0.178， 0.256，0.168，0.196，0.242，0.268，0.157 μg/mL，respectively. RSDs of precision，stability（12 h）and repeatability tests were all lower than 2％（n＝5 or 6）；the recoveries were 97.93％，98.18％，96.57％，97.61％，98.51％，97.45％，98.14％，96.91％（all RSD＜2％ ）. The average contents of albiflorin， paeoniflorin， ferulic acid， naringin， hesperidin， benzoyl guanosine， and glycyrrhizic acid in 6 batches of samples were 59.258，429.237，23.173，625.847，200.424，15.048，67.620 μg/g，respectively. α-cyperone was not detected because of its volatility. CONCLUSIONS：The method is simple，stable，reliable and accurate. It could provide reference for quality control of the lyophilized product of Chaihu shugan san decoction

Combined surgery and sclerotherapy for massive venous malformations of the tongue / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the curative effect of surgery and selerotherapy for massive venous malformations of the tongue.</p><p><b>METHODS</b>From January 2005 to December 2014, subtotal resection or debulking for 15 cases of massive venous malformation in the tongue was undertaken with multiple sessions of pre- and post-operative injection therapy of pingyangmycin, lauromacrogol and absolute ethanol.</p><p><b>RESULTS</b>All signs associated with the lesions including eating, sleep and speech disorders disappeared after treatment. Complete or near complete resolution was achieved in 9 cases, and a significant reduction in size in a further 6 cases after surgical excision and peri-operative sclerotherapy.</p><p><b>CONCLUSIONS</b>For massive venous malformations of the tongue, surgical excision combined with multiple sessions of sclerotherapy is a good treatment option.</p>

Protective effects of resveratrol on acute lung injury induced by lipopolysaccharide in mices / 中国生化药物杂志

Purpose To investigate the effect of resveratrol on acute lung injury induced by lipopolysaccharide (LPS) in mice.Methods Acute lung injury (ALI) was induced by LPS in mice. The changes of lung airway inspiratory resistance (Ri), expiratory resistance (Re), and dynamic lung compliance (Cdyn) were observed with pulmonary function test apparatus. Brochoalveolar lavage fluid (BALF) was collected, and the concentrations of interleukin 1β (IL-1β)、interleukin 6 (IL-6) and tumor necrosis factory α (TNF-α) were measured by ELISA. The ratio of wet to dry weight was calculated to assess lung edema. Pulmonary vascular permeability was examined with injection Evans blue to judge the destructive extent of alveolar epithelial cell and endothelial. Pathological section was made, and the histopathological change was observed with light microscope.Results Resveratrol can inhibit the elevation of Ri and Re, and the descent of Cdyn. Simultaneously, resveratrol reduced the concentration of IL-1β, IL-6 and TNF-α,as well as the wet to dry weight ratio and the pulmonary vascular permeability significantly. Furthermore, it also could attenuate the lung injury on histopathology.Conclusion The results show that pretreatment with resveratrol has a protective effect on ALI induced by LPS. The ultimate inhibiting and release of inflammatory factors were involved in the mechanism of the effects.