RESUMEN
The effect of bee pollen on serum IgM and IgG concentration was investigated in normal and immunodepressed mice.The levels of serum IgM and IgG in normal NIH mice were 0.80? 0.07mg/ml ( X ? SD ) and 11. 42 ?2. 31 mg/ml respectively. The concentration of IgM and IgG were all markedly lowered in immunodepres-ssed mice induced by cyclophosphamide 100 mg/kg/d sc for 4d,hydro-cortison 100 mg/kg/d sc for 6d,60Co ?-irradiation 600 rads and S-180 tumor-bearing in comparison to normal control, while IgG and IgM content in mice treated with bee pollen ( Pollen of Brassica campes-tris L. ) 10 g/kg/d orally for 1 month were significantly increased.The results showed that the stimulating effect of bee pollen on humoral immunity may be the base of beneficial effect in human being.
RESUMEN
The effect of bee pollen of Brassica campestris L. and its alcohol extract on lipid peroxidation was observed in vivo and in vitro.The results showed that the production of lipid peroxides in normal liver hotnogenate of mice and elevation of production of lipid peroxides induced by cysteine and FeSO4 in homogenate were found to be inhibited significantly by in vitro addition of alcohol extract of bee pollen.The elevation of lipid peroxides in serum and liver in adult mice induced by alloxan 75 mg/kg(iv)or by administration of peroxidized corn oil 0.2 ml/mouse was markedly inhibited by oral administration of bee pollen (10 g? kg-1?d-1)for 20 days as compared with respective control groups.The level of lipid peroxide in geriatric mice was also markedly lowered by oral administration of bee pollen (10 g?kg-1?d-1)for 3 months as compared to non-treated geriatric mice.Based on the above in vitro and in vivo experimental results, it may be suggested that bee pollen and its alcohol extract protect tissues against destruction by lipid peroxides.
RESUMEN
Immunoenhancement activity of bee pollen and its acetone extract was studied in normal, sarcoma-180 bearing, cyclophosphamide- and antilymphocyte serum-treated mice.Bee pollen and its acetone extract given orally for 30 days could significantly increase the production of serum anti-SRBC hemolysin (HC50) and the number of spleen plaque forming cells (PFC) in primary response to sheep red blood cell (SRBC) in young and adult mice. The acetone extract of bee pollen could significantly prevent the decrease of HC50) the number of PFC and specific rosette forming cells (SRFC), and the quantitative hemolysin of spleen cells (QHS) against SRBC in S-180 bearing, cyclophosphamide- and antilymphocyte serum-treated mice respectively.These results suggested that bee pollen of Brassica campestris L. and its acetone extract have immune-enhancement activity.