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Objective To evaluate the survival and adverse effects of adjuvant concurrent temozolomid (TMZ) combined with radiochemotherapy in postoperative patients with high grade intracranial glioma.Methods 84 postoperative patients with high grade intracranial glioma were randomly divided into the observation group (42 cases including 25 grade Ⅲ cases and 17 grade Ⅳ cases) and the control group (42 cases including 23 grade Ⅲ cases and 19 grade Ⅳ cases).All patients were treated with concurrent radiochemotherapy after surgical operations,the total radiation dose was 60-66 Gy.The patients in observation group were given daily oral TMZ 75 mg/m2 during radiotherapy.4 weeks after radiotherapy,all of the patients received 6 cycles of TMZ,each cycle lasted 5 days with 28 days interval between each cycles.150 mg/m2 of TMZ was given for the first cycle for 5 days,followed by 200 mg/m2 of drug for the rest of the cycles if no significant drug related toxicities were observed.Results The overall response rates (CR+ PR) were 71.7 ％ (33/46) in the observation group,and 32.6 ％ (15/46) in the control group,and with significant difference between the two groups (P ＜ 0.001).The 1,2 and 3 year survival rates of patients in the observation group were 71.7 ％,47.8 ％,36.9 ％,and 56.5 ％,26.1％,15.2 ％ in the control group.The significant differences were found in 2-year and 3-year survival rates between the two groups (P =0.031,0.018).The median recurrent period in the observation group were 22 and 12 months in the control group,and with singnificant difference (P =0.015).The main side effects were limited to grade Ⅰ or Ⅱ.Conclusions Concurrent TMZ combined with radiotherapy in the treatment of high grade gliomas has better clinical efficacy,and can improve the 2-year and 3-year survival rates.Patients tolerate the strategy well and no severe toxicities are observed.
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Objective To observe the effect of EGFR mutation on radiation induced DNA repair in pulmonary adenocarcinoma ceils.Methods A549 cells with wild-type EGFR and H1975 cells with mutated-type of EGFR were irradiated by 4 Gy of 6 MV X-rays.After irradiation,the formation of nuclear γ-H2AX foci was assayed with immunostaining method,the level of DNA-PKcs-EGFR interaction was detected with coimmunoprecipitation,and nuclear RAD51 expression and EGFR nuclear translocation were detected using Western blot.Results DNA repair in the H1975 cells was significantly lower than that in A549 cells.In the irradiated H1975 cells,there was no EGFR translocation with further nuclear DNA-PKcs binding,and the expression of nucleus RAD51 was not altered.But in the irradiated A549 cells,EGFRDNA-PKcs interaction and nucleus RAD51 were increased.Conclusions Lung adenocarcinoma cell line with mutations in the tyrosine kinase domain (TKD) of EGFR exhibits a high radiosensitivity due to the reduction of the non-homologous end-joining (NHEJ) and homologous recombination (HR) DNA DSB repair kinetics.
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ObjectiveTo detect the efficacy of compound matrine combined with intensity modulated radiotherapy (IMRT) for patients with locally advanced nasopharyngeal carcinoma (NPC). MethodsA total of 96 patients with locally advanced NPC were randomized into compound matrine combined with IMRT group (the therapeutic group) (n =48) and IMRT group (control group) (n =48). The nasopharyngeal DT and lymph nodes Dr were 66-78 Gy/(33-39 f)in 6-8 weeks,60-70 Gy/(30-35 f)in 6-8 weeks, respectively.The prophylactic dose of neck was 50-54 Gy.ResultsThe NPC efficacy of the therapeutic group was 93.8 ％(45/48)and control group was 79.2 ％(38/48).There was significant difference between the two groups in curative effect (P ＜0.05). The lymph nodes efficacy of the therapeutic group was 87.5 ％ (44/48) and control group was 75.0 ％ (30/48). There was significant difference between the two groups in curative effect (P ＜0.05). The side effects of mucosa were fewer in therapeutic group while the infection rate of pharynx oralis was higher in control group (P ＜0.01). ConclusionCompound matrine combined injection can improve shortterm curative effect of IMRT of locally advanced NPC and significantly decreased side effect of radiotherapy.
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Objective To evaluate the technical points and security of CT-guided in percutaneous gold seed fiducials implantation for 132 solid tumors before cyberknife radiosurgery treatment. Methods 132 solid tumors were implanted with gold seed fiducials guided by CT before cyberknife radiosurgery treatment.The complications were analyzed. The methods for the prevention and treatment of the complications were suggested. Results Of 132 cases, the achievement rate of puncture was 99.2%(131/132), including 16 (12.1%) cases of pain in the location of puncture, 10(7.6%) cases of tachycardia, and 6 (4.5%) cases of hypertension. Among the 68 cases of lung cancer, there were 3(4.4%) cases of slight pneumothorax, 3(4.4%)cases of generous pneumothorax. Among the 50 cases of liver cancer, there were 1 (2.0%) case of gold seed fiducials transmigration, 2 (4.0%) cases of small amount bleeding from needle channel. Conclusion Percutaneous gold seed fiducials implantation in CT-guided for solid tumors before cyberknife radiosurgery treatment is a slight trauma and safe method.
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Objective: The purpose of this study was to quantitatively assess the systematic and random errors in radiation therapy for pelvic tumor patients of different somatotypes and seek an individualized mathematic basis for defining the planning target volume(PTV) and planning organ at risk volume(PRV) in 3 dimensional space.Methods: Thirty patients with pelvic tumor were immobilized by thermoplastic body mask in a spine position and divided into four somatotypes according to their body mass indexes(BMI).CT simulations were performed and digitally reconstructed radiographs(DRR) were generated as the reference image.To define the appropriate PTV-and PRV-margins for intensity modulation radiated therapy(IMRT),inter-fractional set-up errors and intra-fractional organ motions were quantitatively evaluated in the right-left(R-L),anterior-posterior(A-P) and cranial-caudal(C-C) directions.And the frequency of shift in the three directions were observed.Results: For the pelvic tumor patients with different BMIs,the inter-fractional set-up errors of the emaciated,moderate,overweight and obese somatotypes were 1.8,1.8,2.2 and 2.7 mm in the R-L direction,2.2,1.8,3.6 and 2.8 mm in the A-P direction,and 2.4,2.1,2.7 and 2.6 mm in the C-C direction.The intra-fractional organ motions of the four somatotypes were 1.9,1.9,2.3 and 2.5 mm in the R-L,2.1,2.2,2.9 and 3.0 mm in the A-P,and 1.8,1.9,2.2 and 2.5 mm in the C-C direction.Conclusion: In the pelvic irradiation,the definition of PTV and PRV should be individualized.
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Objective: To study the accuracy of MRI-CT image registration in order to find a more effective method of image fusion and a standard for the evaluation of post-operative intra-cranial tumor radiotherapy.Methods: Twenty-four patients with intra-cranial tumor underwent MR T1-weighed sequence and post-operative location CT scan under the same location condition.The trans-axial MR T1-weighed sequence and CT image were registered and the correlation between registration scores and errors was analyzed by the method of Tris-Axes Landmark.The linear registration errors were observed in the three-dimensional space of every patient.Results: An improved linear correlation was found between registration scores and errors(r=-0.985,y=-0.551 3x+5.351 4).The registration met the clinical standard when the scores exceeded 6.61.Conclusion: Tris-Axes Landmark is a preferable registration method to be used in clinical radiotherapy for rapid and precise evaluation of MRI-CT image registration accuracy.
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Objective: To observe differentially expressed genes in rat hippocampus irradiated by 6 MV X-rays during radiotherapy.Methods:After whole brain irradiation of 50 Gy in 25 fractions with 2 Gy per fraction,the capability of learning and memory was investigated in a shuttle box model,then rats were killed and the hippocampus were taken,mRNA from sham irradiation group and irradiation group were reversely transcribed to cDNA with incorporation of fluorescence dUTP(Cy5 and Cy3 dUTP) to prepare for hybridization probes.The mixed probes were hybridized to the cDNA microarray.Axon Genechix 4000 BandGenePix Pro3.0 software was used to scan and analyze the fluorescent signals. Results:①The active avoidance response of irradiated rats were lowered significantly.②Compared with the sham group,significant changes took place in 43 genes,among which 28 genes were upregulated and 15 down regulated,other genes did not change significantly.③Among the 43 malexpressed genes,16 were reported previously,others were newly found genes. Conclusion: The influence of ionizing radiation on learning and memory was a complex course,including many transduction factors and might be related to mitochondrial dysfunction.
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Background and purpose:Accurate target delineation in IMRT setting has been a challenge for post-operative gliomas.The fusion technology with MR+CT image registration could improve the accuracy of target delineation,but there are no reports as to whether this technique could increase the stability of target delineation.This study was to investigate the accuracy of different fusion technologies with MRI+CT image registration so it could improve stability of target delineation of clinical target volume in patients with post-operative gliomas.Methods:The post-operative and pre-operative MRI+CT were registrated in nine patients with post-operative gliomas.Clinical target volume in the two registration images and localized CT images were delineated at 5 different times.The image registration accuracy and stability of target delineation were also quantitatively assessed.Results:The registration accuracy in 3D space was always less than 1.5 mm.The concordance index in CT+MRI(pre-matched),CT+MRI(post-matched)and CT(conv)were(68?9)%,(61?7)% and(41?12)% respectively.The same volumes delineated with three sets of images were(82?7)% for CT+MRI(pre-matched),(78?5)% for CT+MRI(post-matched)and(65?8)% CT(conv),respectively.Conclusions:The volume delineated according to pre-operative MRI registered with CT image have the largest volume and the best stability(P