Hederagenin improves psoriasis skin lesions by inhibiting macrophage mincle-mediated inflammation / 中国临床药理学与治疗学

AIM: To observe the effects and mechanisms of hederagenin (HDG) in improving psoriasis skin lesions and inflammation in mice. METHODS: A mouse model of psoriasis was established by depilation of the back and continuous application of imiquimod for 7 days in C57 mice. After modeling, HDG was administered orally (low dose: 25 mg · kg

Identification of Bulbocodin D and C as novel STAT3 inhibitors and their anticancer activities in lung cancer cells / 中国天然药物

Cancer stands as one of the predominant causes of mortality globally, necessitating ongoing efforts to develop innovative therapeutics. Historically, natural products have been foundational in the quest for anticancer agents. Bulbocodin D (BD) and Bulbocodin C (BC), two bibenzyls derived from Pleione bulbocodioides (Franch.) Rolfe, have demonstrated notable in vitro anticancer activity. In human lung cancer A549 cells, the IC50s for BD and BC were 11.63 and 11.71 μmol·L-1, respectively. BD triggered apoptosis, as evidenced by an upsurge in Annexin V-positive cells and elevated protein expression of cleaved-PARP in cancer cells. Furthermore, BD and BC markedly inhibited the migratory and invasive potentials of A549 cells. The altered genes identified through RNA-sequencing analysis were integrated into the CMap dataset, suggesting BD's role as a potential signal transducer and activator of transcription 3 (STAT3) inhibitor. SwissDock and MOE analyses further revealed that both BD and BC exhibited a commendable binding affinity with STAT3. Additionally, a surface plasmon resonance assay confirmed the direct binding affinity between these compounds and STAT3. Notably, treatment with either BD or BC led to a significant reduction in p-STAT3 (Tyr 705) protein levels, regardless of interleukin-6 stimulation in A549 cells. In addition, the extracellular signal-regulated kinase (ERK) was activated after BD or BC treatment. An enhancement in cancer cell mortality was observed upon combined treatment of BD and U0126, the MEK1/2 inhibitor. In conclusion, BD and BC emerge as promising novel STAT3 inhibitors with potential implications in cancer therapy.

Repurposed benzydamine targeting CDK2 suppresses the growth of esophageal squamous cell carcinoma / 医学前沿

Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of ESCC patients. Here, we found benzydamine, a locally acting non-steroidal anti-inflammatory drug, had potent cytotoxic effect on ESCC cells. Benzydamine could suppress ESCC proliferation in vivo and in vitro. In terms of mechanism, CDK2 was identified as a target of benzydamine by molecular docking, pull-down assay and in vitro kinase assay. Specifically, benzydamine inhibited the growth of ESCC cells by inhibiting CDK2 activity and affecting downstream phosphorylation of MCM2, c-Myc and Rb, resulting in cell cycle arrest. Our study illustrates that benzydamine inhibits the growth of ESCC cells by downregulating the CDK2 pathway.

Germplasm resources and secondary metabolism regulation in Reishi mushroom (Ganoderma lucidum) / 中草药·英文版

Ganoderma lucidum is a valuable medical macrofungus with a myriad of diverse secondary metabolites, in which triterpenoids are the major constituents. This paper introduced the germplasm resources of genus Ganoderma from textual research, its distribution and identification at the molecular level. Also we overviewed G. lucidum in the components, the biological activities and biosynthetic pathways of ganoderic acid, aiming to provide scientific evidence for the development and utilization of G. lucidum germplasm resources and the biosynthesis of ganoderic acid.

Research on the characteristics of Tibetan Medicine for treating orthopedics and traumatological diseases based on data mining / 国际中医中药杂志

Objective:To analyze the medication rule of treating orthopedics in Tibetan Medicine by data mining, in order to summarize the characteristics and theory of ethnic minorities medicine for treating orthopedics and traumatology.Methods:By collecting the treatment methods of orthopedics and traumatologic diseases in books of Chinese Materia Medica·Tibetan Medicine Volume and Chinese Medical Encyclopedia·Tibetan Medicine to analyze the frequency, cluster and association rules of Tibetan Medicines by using Office Excel 2019, IBM SPSS Statistics 26.0 and IBM SPSS modeler 14.1 respectively. Results:Among the 202 Tibetan Medicine prescriptions of Orthopedic Department, 338 belongs to Chinese medicines. The top 3 Chinese medicines that are frequently appeared are Chebulae Fructus, Inula racemosa Hook.f, and Carthami Flos. The properties of those medicines are mainly cold, warm and cool, and the tastes are mainly pungent, bitter and sweet; the meridians mainly belong to liver, lung, stomach and spleen; The priscriptions mainly cover four kinds of diseases: trauma, arthralgia syndrome, lumbosacral tendon injury, chest and back tendon injury. The four kinds of cluster combinations were obtained. The core Chinese medicines were Chebulae Fructus, Terminaliae Belliricae Fructus, Phyllanthi Fructus, Cassiae Semen, Olibanum, Abelmoschus moschatus, and the common medicine pair were Chebulae Fructus- Terminaliae Belliricae Fructus, Abelmoschus moschatus- Cassiae Semen, Olibanum- Abelmoschus moschatus and so on. Conclusions:Tibetan Medicines treat orthopedics and traumatological diseases with antipyretic medicines as the main yellow water, followed by blood activating, Qi regulating and wind dampness removing medicines. With Sanguo Decoction and Sanhuang water of Tibetan medicine as the core, they are often combined with other antipyretic and blood cooling medicines and bone connecting medicines. Tibetan Medicine pays attention to the application of dry yellow water theory, which reflects the role of the core theory of Qingxie method and yellow water theory of Tibetan Medicine in the diagnosis and treatment of orthopedic and traumatologic diseases.

Ambient radiation of patients undergoing 18F-FDG PET/CT myocardial metabolism imaging / 中国辐射卫生

Objective To study the ambient radiation of patients undergoing 18F-FDG PET/CT myocardial metabolism imaging, and to provide data for protection of surrounding people against radiation. Methods A total of 33 patients were selected for 18F-FDG PET/CT myocardial metabolism imaging. Dose equivalent rate was measured at the height of patient chest, in different directions, at different time points and at different distances, to investigate the distribution of ambient radiation of the patient. Results At the same time point and distance, the dose equivalent rates at the left and right sides of the patient were lower than the front and back sides. The dose equivalent rate at 1 m in front of the patient was 13-21 μSv/h after 18F-FDG injection, and decreased to 5-14 μSv/h after PET/CT imaging, with a mean decrease of 46%. The ambient dose equivalent rate decreased exponentially with distance (10~300 cm), and the mean power was −1.2. Conclusion The ambient radiation of patients undergoing 18F-FDG PET/CT myocardial metabolism imaging was high after 18F-FDG injection, and the ambient dose equivalent rate decreased rapidly with time and distance. Our results suggest that patients undergoing myocardial metabolism imaging should avoid prolonged and close contact with other people on the day of examination.

Drinking behaviors among different time periods during spring in college students in Hebei Province / 中国学校卫生

Objective@#To investigate drinking behaviors among college students in Hebei Province during 8 periods of a day(before breakfast, during breakfast, after breakfast, during lunch, after lunch, during super, after super, during night in spring, and to analyze the drinking behaviors during different time periods so as to provide the scientific reference for health progaganda of water drinking.@*Methods@#A total of 156 college students in Hebei Province were selected using simple random sampling method. The information of frequency, types and patterns of daily water drinking during 8 time periods, including morning, afternoon and evening, during meal and non-meal time, were recorded using the 7-day 24-hour recording method.@*Results@#During 8 time periods, the amount (325 mL) and the percentages (89.8%) of drinking water after dinner was highest(Z=774.72, P<0.01), while water drinking after lunch and dinner was more common(Z=839.05, P<0.01). The amount (439 mL) of water intake in the evening was highest(Z=45.14, P<0.01), while water intake during the afternoon and evening was more common(2.3 time on average)(Z=40.34, P<0.01). About 54.2% of participants drank water during meal, while 99.2% drank water during non-meal time. The amount of water intake, the percentage of total daily water intake and the number of water intake during meal time was lower than those during non-meal time(Z=-14.58, -15.07, P<0.01).@*Conclusion@#Drinking behaviors in different time period among college students in Hebei Province during spring varies significantly. The amount of water intake in non-meal time is generally higher than that in meal time. Further efforts are needed to increase drinking water availability and consumption, as well encourage consumption of water among college students.

Weekday and weekend drinking behaviors during spring among college students in Hebei Province / 中国学校卫生

Objective@#To investigate drinking behaviors in weekdays and weekends among college students in Hebei Province during spring, and to provide references for propaganda of water drinking.@*Methods@#A total of 156 college students in Hebei Province were selected using simple random sampling method. The survey began in March 2017. 7-day 24hour recording method was applied to record the amount and type of water drinking during for consistent consecutive 7 days, duplicate portion method was used to weigh the food consumed during the consistent consecutive 3 days and to record the water contained in the food.@*Results@#Water intake from food among subjects during weekdays was higher than that among subjects during weekends (1 191 mL vs 1 113 mL, P<0.01). Only 21.9% and 19.9% of subjects consumed sufficient water during weekdays and weekends, according to the recommendation of China on total fluids intake, respectively. The proportion of water intake from staple food among male subjects during weekdays was lower than that during weekends (28.6% vs 31.1%). The proportion of water intake in males from porridge, water intake from soup and vegetables among subjects during weekdays were higher than those among subjects during weekends (6.8% vs 0; 141 mL vs 0 mL; 699 mL vs 647 mL). Water intake from staple food and vegetables among females during weekdays was higher than that during weekends (256 mL vs 210 mL; 559 mL vs 536 mL). There were no statistically differences on plain water, milk and beverages among subjects during weekdays and weekends(P>0.05).@*Conclusion@#Water intake from food among college students in Hebei Province during weekdays in spring is higher than that during weekends. There is discrepancy on sources of water intake from food during weekdays and weekends, while no similar difference on sources of drinking water. Proportion of college students without sufficient daily water intake during weekdays and weekends is relatively high which warrants further notice.

Preparation, characterization and in vitro evaluation of poly(2-ethyl-2-oxazoline) modified curcumin liposomes / 中国生化药物杂志

Objective To prepare Curcumin liposome (Cur-L) and poly(2-ethyl-oxazoline)-cholesteryl methyl carbonate (PEOz-CHMC) was used to modified Cur-L and to evaluate their associated properties in vitro.MethodsEncapsulation efficiency and particle size were taken as evaluation indicators to optimize the formulation and preparation conditions of Cur-L by orthogonal test.The EE, particle size and shape of the liposomes were determined by sephadex G-50 mini-column centrifugation method, ZLS dynamic light scattering instrument and transmission electron microscopy (TEM), respectively.The release of the liposome in vitro was detected by The dialysis method.MTT assay was used to determine the cell inhibition of two Cur-L.ResultsThe optimized preparing method of Cur-L is as following: 1.56(w/w) as drug-lipid ratio, 5.1(w/w) as the ratio of mass of phosphatide and cholesterol, 7.4 as the pH of PBS buffer.The EE of Cur-L was (75.05±0.64)%, while the modification of PEOZ hasno influences on EE.Through TEM, PEOZ-Cur-L has aobviouslipid bilayer structure.The average particle diameter of PEOZ-Cur-L was 84.89 nm.In vitro release experiments showed that in 24h, the accumulative release rate of Cur-L is more than 70% with pH 7.4, while that of PEOZ-Cur-L was less than 25%.The cytotoxicity experiment showed that PEOZ-Cur-L can inhibit HCT116 Human colon cancer cells more effectively.ConclusionThe optimized preparing method of Cur-L is reasonable.PEOZ can provide stability to liposomes well and does not hamper its inhibitive effects.

Effect evaluation of evidence-based nursing for improvement of unhealthy emotion and treatment compliance of patients undergoing painless gastroscopy / 中国实用护理杂志

ObjectiveTo discuss the effect of evidence-based nursing in improvement of unhealthy emotion and treatment compliance of patients undergoing painless gastroscopy. Methods134 patients from May 2008 to December 2010 undergoing painless gastroscopy were chosen as study object.According to voluntariness of the patients and their families,they were divided into the evidence-based nursing group (70 cases) and the routine psychological care group (68 cases).The satisfaction degree with nursing,unhealthy emotion,and the compliance during treatment process were evaluated with SAS and SDS.ResultsThe satisfaction degree of patients in the evidence-based nursing group was 92.86％,higher than 79.41％ in the routine psychological care group.No significant difference was shown in SAS and SDS score before treatment between two groups,but unhealthy emotion and treatment compliance after treatment significantly improved,and the improvement degree of the evidence-based nursing group was more evident. ConclusionsApplication of evidence-based care model in painless gastroscopy has more obvious advantages in improvement of unhealthy emotions and treatment compliance compared with routine nursing.It has more important value for clinical practice and is more conducive to improve the quality of life of patients.