RESUMEN
AIM To establish an HPLC-ELSD method for the simultaneous content determination of euscaphic acid,tormentic acid,crategolic acid,corosolic acid,ophiopogonin D,ophiopogonin D',methylophiopogonanone A and methylophiopogonanone B in Shenmai Zhisou Syrup (Eriobotryae Folium,Ophiopogonis Radix,Glehniae Radix,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ Alltima C18 column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of (acetonitrile-methanol)-0.5％ ammonium acetate flowing at 0.7 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.85％-99.43％ with the RSDs of 0.57％-1.33％.CONCLUSION This simple and accurate method can be used for the rapid quality control of Shenmai Zhisou Syrup.
RESUMEN
AIM To establish an HPLC method for the simultaneous content determination of arctiin,arctigenin,forsythoside A,narirutin,naringin,rhoifolin,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Wuli Huichun Pills (Saposhnikoviae Radix,Arctii Fructus,Forsythiae Fructus,etc.).METHODS The analysis of 70％ methanol extract of this drug was performed on a 25 ℃ thermostatic Agilent TC-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-0.4％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner.RESULTS Eight constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 96.55％-99.50％ with the RSDs of 0.54％-1.72％.CONCLUSION This accurate,reliable and reproducible method can be used for the quality control of Wuli Huichun Pills.