RESUMEN
<p><b>AIM</b>To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action.</p><p><b>METHODS</b>MTT assay, immunohistochemical method and Western blotting were adopted.</p><p><b>RESULTS</b>IL (0.03 - 3 micromol x L(-1)) could inhibit the CASMCs proliferation induced by Phen (0.1 micromol x L(-1)) in a concentration-dependent manner. IL (0.1 micromol x L(-1)) antagonized Phen-induced overexpression of PDGF-beta and bFGF from 0.545 +/- 0.026 and 0.47 +/- 0.03 to 0.458 +/- 0.019 and 0.376 +/- 0.017 (P < 0.01 , P < 0.01). IL (0.1 micromol x L(-1)) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57 +/- 0.04, 0.44 +/- 0.04 and (173 +/- 36)% to 0.46 +/- 0.05, 0.372 +/- 0.021 and (115 +/- 35)% respectively (P < 0.01, P < 0.01, P < 0.01).</p><p><b>CONCLUSION</b>IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-beta, bFGF), protooncogene (c-fos, c-myc) and hsp70.</p>
Asunto(s)
Animales , Proliferación Celular , Células Cultivadas , Vasos Coronarios , Biología Celular , Relación Dosis-Respuesta a Droga , Factor 2 de Crecimiento de Fibroblastos , Metabolismo , Proteínas HSP70 de Choque Térmico , Metabolismo , Isoquinolinas , Farmacología , Músculo Liso Vascular , Biología Celular , Nelumbo , Química , Fenilefrina , Plantas Medicinales , Química , Proteínas Proto-Oncogénicas c-fos , Metabolismo , Proteínas Proto-Oncogénicas c-myc , Metabolismo , Proteínas Proto-Oncogénicas c-sis , Metabolismo , PorcinosRESUMEN
Objective To study the effects of russel viper venom X(RVV X)on blood coagulation protein.Methods We divide diluted protein into control and RVV-X groups,then use chromogenic substract assay to detect the activation effect of RVV-Ⅹ on coagulation factor Ⅶ,Ⅸ,Ⅹ and antithrombin,plasminogen,with or without activator.Results In RVV-Ⅹ group,the coagulation factor Ⅶ, Ⅸ and plasminogen displayed weakly enhanced chromogenesis,all P