Orexin-A in hepatic reperfusion-induced liver injury in rats / 中南大学学报(医学版)

OBJECTIVE@#To explore the change of orexin-A expression in hepatic reperfusion and their association with liver injury, and to find out the role of orexin-A in traumatic stress responses.@*METHODS@#A 70% hepatic reperfusion model of rats was established, setting groups of sham-operation and injury ones with different reperfusion time. A self-produced radioimmunoassay and relevant kits were used to detect the protein level of orexin-A in the plasma and the hypothalamus, serum glucose, total anti-oxidation capacity and alanine transaminase, HE staining and immunohistochemistry were used to investigate the pathological variation and protein expression of orexin-A in the liver, while RT-PCR was applied to observe mRNA expression of orexin-A in the hypothalamus and the liver.@*RESULTS@#Both the shape of standard curve and metrical results of the self-produced orexin-A radioimmunoassay were good. Protein levels of orexin-A in the plasma and the hypothalamus in each reperfusion group showed no significant change. Serum glucose and total anti-oxidation capacity increased significantly at the later phase of injury. There was significant and positive linear correlation between the plasma orexin-A and serum glucose and total anti-oxidation capacity; serum alanine transaminase in each reperfusion group was significantly higher, and liver damage was significantly alleviated at the later phase of the injury. Different extents of variation were observed in protein expression of orexin-A in the liver and its mRNA expression in the hypothalamus and the liver.@*CONCLUSION@#Orexin-A undergoes significant changes during hepatic reperfusion, indicating that orexin-A participates in the modulation of hepatic reperfusion-induced liver injury and internal disorders.

Study on the Relationships between Leptin Levels and Weights of Mothers and Infants and the Relationships of Cord Serum Leptin to C-peptide, Insulin and Insulin Like Growth Factor-II / 中华妇产科杂志

Objective To determine the relationships between serum leptin levels and maternal weights in late pregnancy and cord blood leptin levels to birth weights, C peptide, insulin and insulin like growth factor (IGF II). Methods Fifty normal pregnant women at 37～38 weeks and their newborns were studied, and 29 non pregnant women were set as control. Venous blood was taken from women and from the cord at delivery. Blood leptin and cord blood C peptide, insulin, and IGF II were measured by radio immunoassay. Results The average leptin level in maternal sera was (13.62?3.68)?g/L, significantly higher than that in the control (6.60?3.04)?g/L and that in cord blood (8.05?4.61)?g/L. Maternal leptin levels were significantly correlated with maternal weights and body mass index (BMI. r =0.33, 0.35, P 0.05). Cord blood leptin levels were significantly correlated with birth weights and BMI ( r =0.54, 0.49, P 0.05). Significant difference of the cord leptin levels was not seen between the males and females. The cord blood C peptide was (0.86?0.35) ?g/L, insulin (8.49?4.76) mU/L and IGF Ⅱ(0.218?0.076) ?g/L. Cord leptin levels were correlated with C peptide levels ( r =0.37, P 0.05). Conclusions Maternal leptin levels in late pregnancy were significantly higher than those in normal non pregnant women and positively correlated with maternal weights and BMI. Cord blood leptin levels were positively correlated with birth weights and BMI of the newborns. The leptin levels of cord blood were correlated with those of C peptide but not insulin and IGF II.

Influence of leptin on intestinal function and its protective effect on hepatic and renal functions after sepsis / 中国病理生理杂志

AIM: To detect the effect of sepsis on hepatic,renal functions and corresponding enzymes in intestine of mice,and to explore the role of leptin in acute inflammation.METHODS: A mice model of sepsis was made by cecum ligation and perforation,and 96-well spectrophotometry was used to detecte the levels of alanine aminotransferase(ALT),uric acid(UA) and activities of myeloperoxidase(MPO),glutathin-S-transferase(GST),xanthine oxidase(XOD),superoxide dismutase(SOD) in serum and intestinal homogenized fluids,respectively.Hematoxylin-eosin staining was used simultaneously to check the histopathologic changes of intestine.RESULTS: Compared with sham group(330.12 ?mol/L?94.15 ?mol/L),serum UA level(521.92 ?mol/L?91.86 ?mol/L) at 6 h after sepsis was significantly higher.12 h after sepsis,both serum ALT(83.55 U/L?40.44 U/L) and UA(474.03 ?mol/L?75.22 ?mol/L) were significantly higher than those in sham group(66.23 U/L?16.80 U/L and 320.95 ?mol/L?99.14 ?mol/L,respectively).12 h after leptin injection(0.1 mg/kg,ip) or indomethacin injection(2 mg/kg,ip),the serum ALT and UA levels significantly decreased(vs sepsis group,P

The inhibition of IL-1? and IL-6 release in LPS induced Hela cells by cPLA_2 antisense oligonucleotide / 中国免疫学杂志

Objective:To investigate the effect of cPLA 2(cytosolic PLA 2, 85-kD PLA 2) on the signaling pathways of IL-1?、IL-6 release in LPS induced Hela cells.Methods:①LPS stimulated Hela cells were exposed to the complex of cPLA 2 initiation site-directed antisense oligonucleotide and liposome in the indicated doses and time, and then,analyzed the changes of cPLA 2 both in mRNA and in protein by RT-PCR and Western blot.②At the same time, the supernatants were collected to detect the level of IL-1? and IL-6 under radioimmunoassay(RIA).Results:①The transfection led to a dose-dependent decrease in cPLA 2 protein, while the change of mRNA is not notable.②The levels of IL-1?、IL-6 in the transfected cells were reduced following the repression of cPLA 2 compared with that of LPS added only.Conclusion:cPLA 2 probably plays an important role in the signaling mechanisms on IL-1?、IL-6 release in LPS-induced Hela cell. [