RESUMEN
Objective:To make a magnetic separator and verify its application effect in the removal of foreign bodies in laparoscopic surgery.Methods:From February 2015 to September 2019 in Chengdu Sanliu Third Hospital Affiliated to Southwest Medical University, 46 surgical patients to be searched for during the laparoscopic surgery, such as needle breakage, needle drop, and titanium clip shedding, were selected as the research object. The observation group used a self-made laparoscope with a magnetic separator, and the control group used traditional methods, such as: laparoscopy to flip the tissue and C-arm X-ray positioning. The time to find the foreign body left after surgery, the incidence of postoperative subcutaneous emphysema, and the satisfaction of the surgeon were compared between the two groups.Results:The time to find the foreign body left after surgery was (15.36±3.62)min in the observation group and (75.83±10.30)min in the control group, there was significant difference( t value was 29.384, P<0.01). The incidence of postoperative subcutaneous emphysema was 13.70% (3/22)in the observation group and 83.30%(20/24)in the control group, there was significant difference( χ2 value was 37.079, P<0.01). The satisfaction of the surgeon was 100.0%(22/22)in the observation group and 16.7%(4/24)in the control group, there was significant difference( χ2 value was 48.765, P<0.01). Conclusions:For patients with foreign bodies left after laparoscopic surgery, the use of magnetic separators can significantly shorten the time to find foreign bodies, reduce the incidence of postoperative subcutaneous emphysema, and increase the satisfaction of the surgeon.
RESUMEN
Objective To investigate the detection and clinical significance of thrombus molecular markers in diffuse large B-cell lymphoma (DLBCL). Methods We collected the blood specimens of 60 patients with DLBCL, involving 23 cases in the initial treatment group, 24 cases in the remission group and 13 cases in the non-remission group, 23 cases in the thrombus group and 37 cases in the non-thrombus group. We selected 46 healthy people in the same period as the control group. The levels of thrombomodulin (TM), plasmin-α2 plasmin inhibitor complex (PIC), tissue plasminogen activator-plasminogen activator inhibitor-1 complex (t-PAIC) and thrombin-antithrombin Ⅲ complex (TAT) in plasma were detected by chemical immunoassay, and the levels of lactate dehydrogenase (LDH) in serum was detected by automatic biochemical analyzer. We analyzed the differences of thrombus molecular markers among groups and prognostic factors. Results The levels of TM and PIC in plasma of lymphoma patients were higher than those in health control group (P < 0.05). The levels of TM and PIC in the initial treatment and non-remission groups were significantly higher than those in the remission group (P < 0.05). The levels of TM, PIC and TAT in thrombus group were higher than those in non-thrombus group (P < 0.05). TM and PIC levels in plasma were closely related to the prognosis of DLBCL patients. PIC was an independent prognostic factor (P < 0.001). TM and PIC levels were correlated with LDH prognostic indicators in lymphoma patients. Conclusion TM and PIC levels in plasma are significantly increased in DLBCL patients. They are expected to be the indicators for effectiveness and prognosis of DLBCL patients.
RESUMEN
The integration of the biochemistry teaching content in thematic teaching pattern is the key link to realize the specialization of biochemistry and the theme of teaching contents.Around the theme of the content integration,weakening discipline consciousness and serving the professional ability are the requirements of curriculum reform of the new era and the requirements of the education concept students as the main body,teachers as the leading.In practice,we should conduct content integration three-dimensional perspective fromthree-dimensional perspective three dimensional perspectives of biochemicalthreedimensional perspective knowledge,professional and practical characteristics,including the integration of professional theme and the integration of teaching theme in order to build a suitable subject teaching mode,to further improve the teaching effect of biochemistry.
RESUMEN
Objective To study the protective effect of phenethyl alcohol glycosides extracted from Herba Cistan-chis on human sperm DNA with oxidative damage by Raman spectroscopy. Methods The human sperm model of oxidative damage was induced with Fenton’s reagent in vitro. After co-cultured with the phenethyl alcohol gly-cosides extracted from Herba Cistanchis ( in the dosage of 0.1, 0.01 and 0.001 μg/mL) , the changes of the sperm nuclear DNA were observed by using confocal micro-Raman spectroscopy. Results The intensity and peaks of the Raman spectra of the human sperm nuclei treated by Fenton’s reagent were changed significantly, and then the changes of intensity and peaks were inhibited after treatment with phenethyl alcohol glycosides of Herba Cistanchis, the inhibition being dose-dependent. Conclusion The phenylethyl alcohol glycosides ex-tracted from Herba Cistanchis have protective effect on human sperm DNA with oxidative damage.
RESUMEN
Objective This study seeks to use a meta-analytical approach to quantitatively assess the results of applying the problem-based learning(PBL) teaching model and the traditional lecture-based learning(LBL)teaching model to basic medical courses in undergraduate medical edu-cation. Methods The CNKI and VIP databases were electronically searched to retrieve randomised controlled trial studies that examined the use of PBL methods for basic medical courses in under-graduate medical education. In these studies PBL teaching model was used in experiment group and LBL teaching model was used in control group. Pass rate or test scores was used to evaluate the effect of learning. The retrieved documents ranged from the time that each database was first constructed to December 2012. After two researchers performed literature screening independently, data extraction, and quality assessment procedures in strict accordance with the inclusion and exclusion criteria of this study, the Stata 11.0 software package was utilized to conduct the meta-analysis. Results This meta-analysis examined 28 studies that included a total of 3703 subjects. The modified Jadad scores of 20 studies(71.4%) were less than 4, and those of 8 studies(28.6%) were more than or equal to 4. The meta-analysis revealed that compared with the traditional teaching model, the PBL teaching model did not produce improved examination passing rates for a course [relative risk(RR):1.05, 95%confidence interval(CI):(0.99, 1.10), P=0.098] but could improve examination scores for a course [standardized mean difference (SMD): 0.73, 95% CI: (0.51, 0.96), P<0.001]. Conclusion For basic medical courses in undergraduate medical education, compared to LBL teaching, PBL teaching can improve students' test scores but cannot improve students' pass rate. PBL used in basic medical courses has di-versified forms and lacks unified criterion, so there is still a long way to go for the appli-cation of PBL.