RESUMEN
ABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%.
Asunto(s)
Animales , Masculino , Farmacocinética , Portulaca/clasificación , Extractos Vegetales/análisis , Cromatografía Líquida de Alta Presión/métodosRESUMEN
Objective:To determinate the content of vitexin in Xinan Capsules by RP HPLC.Methods: C 18 ODS(4.6mm?150mm;5?m) column with temperature at 25 ?C was used with a mobile phase of water zspropanol acetic acid(75∶18∶3) elution at the flow rate of 1mL -1 ?min -1 and detection wavelength at 330nm.Results: The linear range of vitexin was from 0.734 to 3.67 ?g, and correlation coefficient was 0.9996 . The content of vitexin in Xinan Capsules is 9.7%. Conclusions:The detection method is simple and convenient, accurate with a good reproducibility. The extraction method of the control sample is simple, and easy to do.