RESUMEN
Objective To observe the effects of Fuzheng Qingfei Decoction combined with Western medicine basic treatment in pulmonary infection of AIDS patients.Methods A total of 154 cases of AIDS pulmonary infection treated in the Affiliated Hospital of Southwest Medical University from November 2013 to December 2016 were selected.Among them,86 cases in the observation group adopted Fuzheng Qingfei Decoction combined with the Western medicine routine treatment;68 cases in the control group only adopted the Western medicine therapy.The levels of procalcitonin (PCT) and C-reactive protein (CRP),CD4+ T cells count and improvement situation of clinical symptoms in the two groups were observed before and after treatment.Results The CD4+ T cells count,levels of PCT and CRP before treatment had no statistical difference between the two groups (P＞0.05),the levels of PCT and CRP after 7 d treatment in the observation group were lower than those in the control group,the differences were statistically significant (P＜0.05),and the CD4+T cell count after 3-month treatment in the observation group was higher than that in the control group and the PCT and CRP levels were lower than those in the control group,the differences were statistically significant (P＜0.05);the total effective rate in the observation group was 87.21％,which was better than 76.74 ％ in the control group,the difference was statistically significant (P＜0.05).Conclusion Fuzheng Qingfei Decoction combined with routine Western medicine therapy for treating AIDS complicating pulmonary infection can increase the anti-infection effect,reduce the inflammatory response,enhance CD4+ T cells count,relieve the clinical symptoms and improve their living quality.
RESUMEN
Objective To evaluate the relationship between mRNA and protein expression of HtrA and Streptococcus mutans i-solated from the children with different caries experience and to provide the theoretical and experimental basis on prediction of dent-al caries in deciduous teeth .Methods The strains of Streptococcus mutans isolated from children with different carious experiences in the preliminary experiments were divided into three groups :high caries-susceptible group ,middle caries-susceptible group ,caries-free group .All strains were reanimated on the agar plate of MS ,and after smear pure culture examination ,typical bacteria were in-cubated in BHI ,then purified nucleic acid and extracted all the RNA of streptococcus mutans by reverse transcription PCR and de-tected it by agarose gel electrophoresis integrality .Synthetic cDNA and take further PCR amplification with cDNA products .Ob-serve records results by Gel imaging system .HtrA of target gene and electrophoresis image were gray scan by Gel quantitative soft-ware Gel-Pro analyzer 4 .0 was used to analyze relative expression value of gene .After purifying protein ,collected total protein of Streptococcus mutans strains by Western Blot method ,then tested the concentration of total protein sample .The results of Chemilu-minescence imaging were scanned into computer by Bio-Rad analyzing system ,calculated the gray value by software Quantity One 4 .4 .0 which showed the relative expression level of protein .Results There were significant differences in HtrA mRNA and protein expression of different Streptococcus mutans isolated from the children with different caries susceptibility .high caries-susceptible group> middle caries-susceptible group> caries-free group (P< 0 .05) .Conclusion There were significant differences in HtrA mRNA and protein expression of different Streptococcus mutans isolated from the children with different caries susceptibility .The higher caries susceptibility the group was ,the more HtrA mRNA and protein the strain express .