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Acta Pharmaceutica Sinica ; (12): 794-798, 2013.
Artículo en Chino | WPRIM | ID: wpr-259548

RESUMEN

This study is purposed to investigate the effects of praeruptorin A (PA) and praeruptorin C (PC) on UGT1A1 in HepG2 cells through hCAR pathway. PA and PC were incubated with HepG2 cells for 24 h and 48 h, mRNA and protein expressions of UGT1A1 were determined by real-time PCR and Western blotting assays. Additionally, effects of PA and PC on UGT1A1 mRNA and protein expressions were also measured after transient transfection of a specific CAR siRNA for 72 h in HepG2 cells. UGT1A1 mRNA and protein expression levels were significantly increased by PA and PC after incubation for 48 h. Moreover, the mRNA and protein up-regulations of UGT1A1 were attenuated by transient transfection of a specific CAR siRNA, suggesting the induction was mediated by CAR. The results suggest that PA and PC can significantly up-regulate UGT1A1 expression partially via the CAR-mediated pathway.


Asunto(s)
Humanos , Apiaceae , Química , Cumarinas , Farmacología , Medicamentos Herbarios Chinos , Farmacología , Glucuronosiltransferasa , Genética , Metabolismo , Células Hep G2 , Raíces de Plantas , Química , Plantas Medicinales , Química , ARN Mensajero , Metabolismo , ARN Interferente Pequeño , Genética , Metabolismo , Receptores Citoplasmáticos y Nucleares , Genética , Metabolismo , Transducción de Señal , Transfección
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