Expression of Fas and Fas ligand in infiltrating lymphocytes in patients with oral lichen planus / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To examine the expression of Fas and Fas ligand (FasL) in T lymphocytes of oral lichen planus (OLP) and the effects of Fas, FasL and activation-induced cell death (AICD) on OLP.</p><p><b>METHODS</b>The oral mucosa samples from patients with OLP and normal oral mucosa were assessed by in situ terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine-5'-triphosphate (dUTP)-biotin nick end-labelling (TUNEL) assay for nucleosomal DNA fragmentation of apoptotic cells in infiltrating lymphocytes. Immunohistochemical technique was used for detection of expression level of Fas and FasL. Immunohistochemical double labeling was performed to examine the expression of Fas and FasL in CD8+ T cells and CD4+ T cells.</p><p><b>RESULTS</b>The rate of apoptosis in infiltrating lymphocytes of OLP was (1.9 +/- 1.8)%, which was lower than that of normal oral mucosa (P = 0.013). The expression of Fas and FasL were increased in lymphocytes of OLP (P = 0.005 and 0.000 respectively). The positive rate of double labeled cells of CD8+ and Fas+ was not increased in OLP (P = 0.313), and of CD8+ and FasL+ in OLP was higher than that of normal oral mucosa (P = 0.002). The expression of double labeled cells of CD4+ and Fas+ in OLP was higher than that of control group (P = 0.031). The expression of CD4+ and Fas+ T cells in reticular OLP were increased (P = 0.019), and of CD4+ and FasL+ did not show remarkable change (P = 0.097).</p><p><b>CONCLUSIONS</b>There was a low frequency of lymphocytic apoptosis. T cells, especially CD8+ T cells in OLP and CD4+T cells in atrophic-erosive OLP appeared to escape from AICD, which may account for the persistence of inflammation. Some CD4+ T cells in reticular OLP may go through AICD.</p>

Expressions of proliferating cell nuclear antigen, Bcl-2, and Bax in lymphocytes of patients with oral lichen planus / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the expressions of proliferating cell nuclear antigen (PCNA), B cell lymphoma-2 (Bcl-2) and Bcl-associated X (Bax) in the lymphocytes of patients with oral lichen planus (OLP).</p><p><b>METHODS</b>Immunohistochemistry was used for detecting the expressions of PCNA, Bcl-2 and Bax proteins in oral lichen planus tissue and normal oral mucosa (NOM).</p><p><b>RESULTS</b>The expressions of PCNA and Bcl-2 proteins increased significantly in the lymphocytes of patients with OLP compared with those in NOM. The expressions of PCNA and Bcl-2 proteins showed no significant differences between patients with OLP of general type and erosive type.</p><p><b>CONCLUSION</b>Increased expression of PCNA and the imbalance of Bcl-2/Bax expressions in the lymphocytes of patients with OLP can be important causes for continuous lymphocyte infiltration in OLP.</p>

Expression of TGF-beta receptors in CD8+ T cells of oral lichen planus / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the expression of TGF-beta receptors in CD8+ T cells of oral lichen planus (OLP).</p><p><b>METHODS</b>Immunohistochemical double labeling technique was used to examine the expression of TGF-betaR I and TGF-betaR II in CD8+ T cells of 28 OLP patients and in 10 controls. The results were compared between the two groups.</p><p><b>RESULTS</b>The double labeled cells were negative in controls. The positive rates of double labeled cells of CD8+/TGF-betaR I and CD8+/TGF-betaR II in OLP were (8.82 +/- 9.98)% and (1.11 +/- 2.94)% respectively.</p><p><b>CONCLUSIONS</b>The expression level of TGF-betaR II in CD8+ T cells of OLP did not increase compared with the controls, but the expression of TGF-betaR I increased. The results indicate the abnormal TGF-beta signal transduction in CD8+ T cells of OLP, which may contribute to the persistence of the chronic inflammation in OLP.</p>