Effect and safety of EX - PRESS shunt implantation combined with biological amniotic membrane implantation for primary open angle glaucoma / 国际眼科杂志(Guoji Yanke Zazhi)

·AIM: To analyze the curative effect and safety in early stage primary open angle glaucoma (POAG) between simple Ex-PRESS shunt implantation and its combination with biological amniotic membrane implantation. ·METHODS: The clinical data of 53 cases for POAG treated with simple EX-PRESS shunt implantation (22 cases,34 eyes) as control group and its combination with biological amniotic membrane implantation (31 cases,38 eyes) as experimental group were retrospectively analyzed. Intraocular pressure (IOP) preoperatively and postoperatively were statistically evaluated. Filtering bleb and incidences of postoperative complications were observed in our hospital during the period of January of 2015 to April of 2017. ·RESULTS: At each time point after the operation, the mean IOP of the experimental group and the control group decreased distinctly respectively, the difference was statistically significant (P< 0. 05). There was no significant difference on the mean IOP between the experimental group and the control group at 1d and 1wk after operation (P<0.05). The mean IOP in the control group was higher than experimental group at 3 and 6mo (P<0. 05). The rate of functional filtration blebs were different between experimental group and control group during at postoperatively 3mo ( P < 0. 05 ). The complications included shallow anterior chamber, hemorrhage, posterior synechia of the iris, and corneal edema after surgery, and its incidences was not different between two groups (P<0.05). · CONCLUSION: EX - PRESS shunt combined with biological amniotic membrane implantation can effectively and safely reduce intraocular pressure, and improve the condition of filtering bleb after operation, and has long-term effect on IOP.

IGF2 expression in blood is not associated with its imprinting status in healthy pregnant Chinese women

Loss of Imprinting (LOI) of IGF2 and over-expressed IGF2 are associated with tumorigenesis. Our previous epidemiological study found a relatively high frequency of IGF2 LOI in healthy mid-gestation pregnant women. The aim of this study is to determine whether the expression of IGF2 is associated with its imprinting status in healthy Chinese pregnant women. The IGF2 imprinting status of 300 pregnant women was analyzed. 20 cases of IGF2 LOI and 20 cases of IGF2 retention of imprinting (ROI) were selected randomly for IGF2 expression analysis. The expression pattern of IGF2 between the group with IGF2 ROI and group with IGF2 LOI in healthy Chinese pregnant women was evaluated by real time PCR and western blot. The result showed no significant differences between IGF2 ROI and LOI groups in mRNA and protein levels. These results imply that IGF2 imprinting status has no obvious impact on its expression. There may be some unknown important factors other than imprinting status driving IGF2 expression.

Jingjielianqiao Decoction promotes leg ulcer healing in rabbits / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the effect of Jingjielianqiao Decoction in promoting leg ulcer in rabbits.</p><p><b>METHODS</b>Nine adult male New Zealand albino rabbits with chronic leg ulcers were randomized into 3 groups, namely group A treated with Jingjielianqiao Decoction, group B with Shengjiyuhong Decoction, and group C with normal saline. Gross observation of the wounds was carried out regularly for evaluating the changes in the ulcerous area, depth and wound surface excretion. After 3 weeks of treatment, the tissues on the edge of the ulcer were sampled and prepared for routine pathological examination, electron microscopy and immunohistochemistry for vascular endothelial growth factor (VEGF) and CD34. The number of blood vessels and their areas were also recorded.</p><p><b>RESULTS</b>The wounds showed no significant differences between the 3 groups by gross observation during the treatment, but after completion of the 3-week treatment, routine pathological examination and electron microscopy revealed significant differences between the groups. Immunohistochemistry for VEGF and CD34 yielded comparable results between groups A and B (positive control), but showed significant differences between group C and the other two groups (P<0.01).</p><p><b>CONCLUSION</b>Jingjielianqiao Decoction and Shengjiyuhong Decoction can obviously promote the healing of leg ulcer in rabbits.</p>