Clinical correlation study of Naomai Jiejing decoction on aneurysmal subarachnoid hemorrhage / 中国药师

Objective To investigate the clinical correlation of Naomai Jiejing decoction with cerebrovascular hemodynamics,thromboelastography(TEG)and rehabilitation outcome in patients with aneurysmal subarachnoid hemorrhage(aSAH).Methods A total of 160 patients with aSAH admitted to Zhongshan Traditional Chinese Medicine Hospital from January 2020 to December 2022 were selected as the study objects,and were divided into study groupand control group according to whether the patients used Naomai Jiejing decoction.The control group was treated with basic therapy combined with nimodipine,the study group was treated with basic therapy combined with nimodipine and Naomai Jiejing decoction(No.1)before surgery,and was treated with basic therapy combined with Nimodipine and naomai Jiejing decoction(No.2)after surgery.Chinese medicine symptom score,cerebrovascular hemodynamics indexes[systolic peak velocity(Vs),mean blood velocity(Vm),end-diastolic peak velocity(Vd),pulsatile index(PI)and resistance index(RI)]and TEG parameters[maximum amplitude(MA),α angle and coagulation index(CI)]were compared between the two groups before and after treatment.Finally,the short-term prognosis of the two groups was evaluated by glasgow prognostic score,and the short-term prognosis of the two groups was compared.Results A total of 160 aS AH patients were included,with 85 in the study group and 75 in the control group.After treatment,the Chinese medicine symptom scores in the study group were significantly lower than those in the control group(P＞0.05).After treatment,the indexes of cerebrovascular hemodynamics in the study group were better than those in the control group(P＜0.05).After treatment,the TEG parameters in the study group were significantly lower than those in the control group(P＜0.05).The overall prognosis of the study group was better than that of the control group,and the proportion of patients with good recovery was significantly higher than that of the control group(P＜0.05).Conclusion Naomai Jiejing decoction has a good treatment effect on aSAH patients,can improve cerebrovascular hemodynamics and TEG parameters,relieve clinical symptoms,and improve the short-term prognosis of patients.

Research progress on work-related musculoskeletal disorders of coal miners / 中国职业医学

Coal miners are a high-risk occupational group of work-related musculoskeletal disorders(WMSDs). This disease not only causes a reduction in the quality of life of workers, loss work ability, and decline in work efficiency, but also becomes an important factor leading to increase social medical burden. At present, most of the evaluation of WMSDs of coal miners at home and abroad adopts the international general scale. Chinese scholars have developed a WMSDs questionnaire that meets the occupational characteristics of Chinese population based on the international general scale—the Chinese Musculoskeletal Disorders Questionnaire. The questionnaire has good reliability and validity, and is a reliable and effective tool for evaluating WMSDs. The prevalence of WMSDs in coal miners is relatively high. The occupational factors that affect WMSDs in coal miners are mainly physical load, postural load, mental load, work organization, and work environment. The individual factors are mainly age, working experience, and education level. It′s recommended to prevent and control the occurrence of coal miners′ WMSDs through multi-level intervention measures such as strengthening education, changing the working posture, and improving the working conditions of coal miners, and rationally arranging labor organizations.

Effect of Astragalus Polysaccharide on Radiosensitivity and Epithelial-mesenchymal Transition of Human Nasopharyngeal Carcinoma CNE-1 Cells / 中国实验方剂学杂志

Objective:Astragalus polysaccharide (APS) was used in combination with ionizing radiation (IR) to investigate the mechanism of APS on the radiosensitivity of human nasopharyngeal narcinoma CNE-1 cells and the epithelial-mesenchymal transition (EMT). Method:Cell counting kit-8 (CCK-8) was used to detect the cytotoxicity of different concentrations of APS (0，6.25，12.5，25，50，100，200 g·L-1) on CNE-1 cells. Colony formation assay was used to calculate the survival fraction (survival fraction, SF) of CNE-1 cells treated with 12.5 g·L-1 APS combined with different radiation doses (0，2，4，6 Gy). The linear quadratic equation mathematical model (LQ) was used to draw the radiosensitivity curve according to SF value. Cell scratch and transwell chamber test were used to detect the migration and invasion ability of cells in each group. The apoptosis of cells in each group was detected by flow cytometry, Western blot was used to detect the expressions of EMT markers, apoptosis markers and protein kinase B/extracellular regulated protein kinases (Akt/ERK) pathway proteins in each group. Result:The results of colony formation assay and radiosensitivity curve showed that the combination of non-toxic dose of 12.5 g·L-1 APS and radiation dose of 4 Gy could significantly increase the radiosensitivity of CNE-1 cells. Compared with blank group and IR group, APS combined with IR could significantly inhibit the migration and invasion of CNE-1 cells (P<0.05), and increase the rate of apoptosis (P<0.05). In addition, compared with the blank group and the IR group, APS combined with IR could significantly down-regulate the expressions of N-cadherin, p-Akt and p-ERK, and significantly up-regulate the expressions of E-cadherin, Bax and Caspase-3 (P<0.05). Conclusion:APS combined with IR can inhibit the migration and invasion of CNE-1 cells, and increase the apoptosis induced by radiotherapy, which may be related to the inhibition of EMT and Akt/ERK pathway.

Analysis of influencing factors on infrared spectroscopic determination of the content of free silica in quartz sand / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To find a suitable method for detecting SiO2 in quartz sand and to analyze the influencing factors on infrared spectroscopic determination of the content of free silica in quartz sand.</p><p><b>METHODS</b>The infrared spectroscopy was used to detect the free silica content of quartz sand, the various factors of influencing the results were analyzed and the control scheme was proposed.</p><p><b>RESULTS</b>The number of particles less than 5 um and the proportion of free silica content increased with the grinding time. When the grinding time was 10-20 min, the results of detecting the free silica content tended to be stable. When the ashing temperature was below 550 degrees C, there was no effect on the free silica content. Although the silica content decreased slightly at ashing temperature 600 degrees C as compared to ashing temperature 550 degrees C, the difference of the free silica content between 550 degrees C and 600 degrees C was not significant (P > 0.05). When the ashing temperature was 600 degrees C, the free silica content in quartz sand samples did not change obviously in 1 h (F = 4.231, P > 0.05). The free silica content in quartz sand samples decreased significantly at 2 h of ashing time, as compared with 2 h of ashing time (F = 10.231, P < 0.05). The average content of free silica was 88.56% +/- 5.75% by pyrophosphate determination, which was significantly higher than that (21.23% +/- 11.25%) by infrared determination (P < 0.05). There was no significant correlation of the average content of free silica between pyrophosphate determination and infrared determination (r = 0.411, P > 0.05).</p><p><b>CONCLUSION</b>The free silica contents detected by pyrophosphate determination were significantly higher than those detected by infrared determination for the same quartz sand samples. It is suggested that the method of detecting the free silica contents in quartz sand samples prefers the pyrophosphate determination to infrared determination.</p>

Endovascular embolization treatment of cerebral arteriovenous malformation with Glubran (NBCA-MS) / 中华神经医学杂志

Objective To explore the clinical skills and curative efficacy of Glubran (NBCA-MS) embolization of cerebral arteriovenous malformations. Methods Ninety-six cases of cerebral arteriovenous malformations were treated by 187 endovascular embolizations with different concentrations of Italy GEM company's Glubran (NBCA-MS). Follow-up was performed to 96 cases of cerebral arteriovenous malformation with NBCA-MS embolization. Results During the follow-up in 96 cases, clinical symptoms completely disappeared in 92, were improved in 3 and reoccurred in 1. The malformation was totally got rid of in 44 of 48 cases DSA examinated, and the 80%-90% embolization was achieved in the rest who need further γ-knife therapy. In all cases, γ-knife therapy was performed in 32 cases after embolization, and among them 26 cases received DSA examination 1-2 years after γ-knife therapy, and 21 cases were proved to have no malformation. Conclusions The method ofendovascular emdolization with NBCA-MS is safe, reliable and effective to treat the cerebral arteriovenous malformation.

Clinical follow-up study of intracranial aneurysm occlusion with expandable hydrocoil / 中华神经医学杂志

Objective To study the short-term and long-term effectiveness of intracranial aneurysm occlusion with expandable hydrocoil. Methods Forty-one patients with intracranial aneurysms (n=45) were treated by endovascular occlusion with expandable hydrocoil. Follow-up interviews in the forms of DSA, CTA or MRA were conducted to the 41 patients within 6-24 months after the treatment to find out the tumor recurrence and complications. Results In the 41 patients, 1 died, 1 suffered from recurrence, 3 developed cerebral infarction, 1 got oculomotor paralysis, 2 got hydrocephalus. According to modified Rankin scale, grade 0 in 8 cases, grade 1 in 19, grade 2 in 7, grade 3 in 3, grade 4 in 2, grade 5 in 1 and grade 6 in 1. Conclusions Endovascular embolization with expandable hydrocoil is an effective treatment method for intracranial aneurysms, especially for parent artery occlusion, but it may be able to cause more complications in the treatment of small aneurysms (＜5mm), so the caution should be taken.

Effects of superconducting knife diameter and freezing time of Endocare CryocareTM Surgical System on frozen necrosis area and histopathological changes of normal canine brain / 中华神经医学杂志

Objective To investigate the effects of the variables of superconducting knife diameter and freezing time of Endocare CryocareTM surgical system on the necrosis extent and micro-pathological changes of the normal frozen brain tissue of dogs. Methods Argon-helium refrigeration superconducting knives of 2 and 3mm in diameter were respectively inserted into the right frontal lobe of dog brains, and the brains were frozen for 3 and 5min, respectively. The procedure was repeated one time. 48h later, the brains were perfused and harvested for the pathological observation under light microscope and electron microscope and the determination of necrosis area. Results 48h after refrigeration, the frozen brains presented hemorrhagic necrosis and were obviously divided into the central necrosis, inflammatory reaction zone, bleeding and edema zone. The boundaries were clear. Survival of brain cell structure was not found in the edge of frozen area. Conclusion Two freeze-thaw cycles with the freezing time of 3 and 5min are enough to cause the death of frozen brain tissue.