RESUMEN
Objective:To develop a RP-HPLC method for simultaneously determining the contents of baicalin,paeoniflorin and pu-erarin in Shengma Huangqin granules. Methods:Three active compounds were chromatographically separated on a Kromasil C18 column (150 mm ×4.6 mm,5 μm) using the isocratic mobile phase composed of acetonitrile-0.1%H3PO4( 20∶80) coupled with changed flow rate and detection wavelength:1-12 min:1. 0 ml·min-1 ,230nm;12. 01-20. 0 min:1. 5 ml·min-1 ,278nm. The temperature of column was 30℃,and the injection volume was 20μl. Results:The linear range of baicalin, paeoniflorin and puerarin was 10-160,30-480 μg·ml-1and 20-320 μg·ml-1 with average recovery of 100. 17%(RSD=1. 29%),100. 14%(RSD=0. 47%) and 100. 68%(RSD=1. 81%),respectively. Conclusion: The method is convenient,accurate and reproducible for the determination of baicalin , paeoniflorin and puerarin in Shengma Huangqin granules.