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Objective:To detect the hypoglycemic drugs illegally added in Haokangshu capsules .Methods: High performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used with an ACQUITY BEH C18 column(50 mm ×2.1 mm, 1.7 μm)and methanol-0.01 mol· L-1 ammonium acetate as the mobile phase with gradient elution .Through the molecular ion peak, the second order mass fragment ion information and MRM mode chromatographic peak retention time and the other information , the cap-sules containing metformin hydrochloride and 11 kinds of chemical hypoglycemic drugs were determined .Results:Through the analysis of 11 chemical hypoglycemic drugs , two chemical hypoglycemic agents , phenformin and glyburide , were detected out in the capsules . Conclusion:Phenformin and glibenclamide are illegally added in Haokangshu capsules .
RESUMEN
Object To develop a method for the identification of Fritillaria walujewii Regel by UV spectrum. Methods UV spectrum of the absolute alcoholic extract of F. walujewii was determined and compared with that of four other similarly treated Fritillaria species. Results The UV spectrum of F. walujewii was consistently different with that of the other four species. Conclusion UV spectrum can be used to differentiate F. walujewii from other species of Fritillaria.