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Objective To assess the functional changes of the hind paw after SD rats being cut offthe L5 spinal nerve by CatWalk gait analysis.Methods Eleven male SD rats were chosen; the right side of these rats,as the experimental side,was cut the ventral root and dorsal root of L5 spinal nerve,while the left side was without any operation.One day after the operation,whether the models were successfully constructed was verified by tracer experiment with wheat germ agglutinin horseradish peroxidase (WGA-HRP) in 3 rats; 1 day before the operation and 3 months after the operation,the changes of such parameters as general parameters,individual paw parameters,pain-related parameters and coordination-related parameters were assessed by using CatWalk gait analysis.Results The WGA-HRP-labeled motoneurons and fibers were not found in posterior horn and anterior horn of the experimental side of L5 spinal gray matter,but both located in the left side,indicating that the models were constructed successfully.General parameters,individual paw parameters and pain-related gait parameters could be recorded 3 months after the operation,but no statistically significant difference was noted as compared with those 1 day before the operation; however,significant decrease of values of regularity index (RI) and coupling diagonal right after-left front in rats 3 months after the operation was noted as compared with those 1 day before the operation (P＜0.05).Conclusion Only a part of coordination-related gait parameters (the lower limbs) is influenced after being cut L5 spinal nerve;therefore,L5 spinal nerve can be used for constructing of a bladder reflex arc.
RESUMEN
Objective To compared the therapeutic effect of selective posterior rhizotomy(SPR)and selective posterior anastomosis(SPA)on children with cerebral palsy.Methods The postoperative follow-up was carried out in 96 patients with cerebral palsy,including 47 children performed bilateral SPR from L_3 level to S_1 level and 49 children performed SPA from the distal ends of L_3 level to L_5 level and from L_4 level to S_1 level,respectively.The tension and myotatic reflex of the lower limbs were estimated 2 w and 1 y after operation.Results Follow-up was conducted for 12 to 18 months on these patients.No significant difference on tension and myotatic reflex was found between patients performed SPR and that performed SPA 2 w after the operation(P>0.05).No significant difference of muscular tension,but statistic difference of motor function in the patients performed SPA was showed as compared with that in the patients performed SPR 1 y after the operation(P>0.05).Conclusion Long-term follow-up shows great motor function improvement on patients performed SPA.