prevalence of coronavirus in fecal samples of neonatal calf diarrhea using electron microscopic examination

Neonatal calf diarrhea [NCD], also known as calf scours, is caused by viruses, bacteria, and parasites. Coronavirus is one of the important causes of NCD syndrome. Electron microcopy has been used for demonstration of viruses in fecal samples of diarrheic calves since 1969. The aim of this study was to determine the prevalence of coronavirus in fecal samples of neonatal calf diarrhea using electron microcopy examination of fecal samples. For the purpose of this study, a total of 100 cases [under 2 months of age] of diarrheic calves from 25 commercial farms in four districts of East Azerbaijan province of Iran were used. Fresh Fecal samples using sterile swab were collected from every calf with clinical diarrhea. All samples were transferred into a sterile container and then were sent to the laboratory to be kept in -20oC freezer until examination. Electron microscopic examination was applied to all fecal samples. Chi-square test was used to analyze the data. According to the results of this study, coronavirus was present in 15% of cases and a significant difference was found between the prevalence rate and the seasons [p<0.05]. The highest prevalence was recorded in winter [28%] and the least prevalence was seen in summer [4%]. There was not any significant difference in both age groups and different districts according to the prevalence rate of infection. Neonatal calf diarrhea is a multi-etiological syndrome. Among the viral diarrhea, bovine coronavirus is one of the major causes of this syndrome. For detection of coronavirus in feces, electron microscope examination is a gold standard diagnostic technique. It seems that coronavirus can be considered as one of the important etiologies of neonatal calf diarrhea in east Azerbaijan of Iran

Sonographic evaluation of the relationship between rheumatoid arthritis and renal stones

Considering the different renal manifestations of rheumatoid arthritis [RA], we carried out this study to investigate the prevalence of renal stones in patients with RA compared to general population. From January 2000 to March 2001, 74 patients with RA and 58 healthy individuals as the control group were evaluated for kidney stones with sonography. All patients were diagnosed on the basis of 1987 criteria of American College of Rheumatology [ACR].1 In the group of patients with RA, 6 out of 74 subjects [8.1%] had renal stones. The prevalence of renal stones in the control group was 8.6% [5 out of 58 persons]. The difference in the incidence of renal stones in the two groups was not statistically significant. The size of renal stones in the two groups was not significantly different. We did not find any relationship between the duration of RA or its various symptoms and the incidence of renal stones. Although RA involves kidneys and in previous studies it was mentioned that the incidence of renal stones in the patients with RA is higher than general population, we did not find any significant statistical correlation between RA and the incidence of renal stones in our study