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Chinese Journal of Experimental and Clinical Virology ; (6): 489-491, 2012.
Artículo en Chino | WPRIM | ID: wpr-305000

RESUMEN

<p><b>OBJECTIVE</b>To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon alpha1b.</p><p><b>METHODS</b>Mouse monoclonal antibodies with different binding site on rIFN-alpha1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively. And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit, specificity, accuracy and reproducibility were evaluated and validated.</p><p><b>RESULTS</b>The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml, with a liner detection range 10-100 ng/ml (R2 = 0.992), variation coefficient inter-plates is less than 10%.</p><p><b>CONCLUSION</b>The developed sandwich ELISA was a sensitive and specific, accuracy and reproducibility method for quantitative determination of recombinant human interferon alpha1b in final product.</p>


Asunto(s)
Animales , Humanos , Ratones , Anticuerpos Monoclonales , Ensayo de Inmunoadsorción Enzimática , Métodos , Interferón-alfa , Sangre , Ratones Endogámicos BALB C
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